Multiple Site-Specific One-Pot Synthesis of Two Proteins by the Bio-Orthogonal Flexizyme System

被引：2
|
作者：
Xiao, Qiuyun [1 ,2 ]
Liu, Zihan [1 ]
Zhao, Xuan [1 ]
Xiong, Hai [1 ]
机构：
[1] Shenzhen Univ, Inst Adv Study, Shenzhen, Peoples R China
[2] Shenzhen Univ, Coll Optoelect Engn, Key Lab Optoelect Devices & Syst, Minist Educ & Guangdong Prov, Shenzhen, Peoples R China
来源：
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY | 2020年 / 8卷
关键词：
genetic encoding; post-translational modifications; cell-free translation; flexizyme; acetyl-lysine; histone; GENETIC-CODE; HISTONE; CROSSTALK; CHROMATIN; LYSINE;
D O I：
10.3389/fbioe.2020.00037
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Lysine acetylation is a reversible post-translational modification (PTM) vastly employed in many biological events, including regulating gene expression and dynamic transitions in chromatin remodeling. We have developed the first one-pot bio-orthogonal flexizyme system in which both acetyl-lysine (AcK) and non-hydrolysable thioacetyl-lysine (ThioAcK) were site-specifically incorporated into human histone H3 and H4 at different lysine positions in vitro, either individually or in pairs. In addition, the high accuracy of this system moving toward one-pot synthesis of desired histone variants is also reported.
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页数：6
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