Multiple Site-Specific One-Pot Synthesis of Two Proteins by the Bio-Orthogonal Flexizyme System

被引：2

作者：

Xiao, Qiuyun ^{[1
,2
]}

Liu, Zihan ^{[1
]}

Zhao, Xuan ^{[1
]}

Xiong, Hai ^{[1
]}

机构：

[1] Shenzhen Univ, Inst Adv Study, Shenzhen, Peoples R China

[2] Shenzhen Univ, Coll Optoelect Engn, Key Lab Optoelect Devices & Syst, Minist Educ & Guangdong Prov, Shenzhen, Peoples R China

来源：

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY | 2020年 / 8卷

关键词：

genetic encoding; post-translational modifications; cell-free translation; flexizyme; acetyl-lysine; histone; GENETIC-CODE; HISTONE; CROSSTALK; CHROMATIN; LYSINE;

D O I：

10.3389/fbioe.2020.00037

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lysine acetylation is a reversible post-translational modification (PTM) vastly employed in many biological events, including regulating gene expression and dynamic transitions in chromatin remodeling. We have developed the first one-pot bio-orthogonal flexizyme system in which both acetyl-lysine (AcK) and non-hydrolysable thioacetyl-lysine (ThioAcK) were site-specifically incorporated into human histone H3 and H4 at different lysine positions in vitro, either individually or in pairs. In addition, the high accuracy of this system moving toward one-pot synthesis of desired histone variants is also reported.

引用

页数：6

共 41 条

[1] Site-Specific One-Pot Dual Labeling of DNA by Orthogonal Cycloaddition Chemistry
Schoch, Juliane
Staudt, Markus
Samanta, Ayan
Wiessler, Manfred
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BIOCONJUGATE CHEMISTRY, 2012, 23 (07) : 1382 - 1386
[2] Chemoenzymatic Bio-orthogonal Chemistry for Site-Specific Double Modification of Recombinant Thrombomodulin
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Wang, Lin
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[3] Noncanonical amino acids as doubly bio-orthogonal handles for one-pot preparation of protein multiconjugates
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Su, Yeyu
Yu, Yutong
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Wang, Haoyu
Shi, Xiaomeng
Wang, Jing
Yang, Xing
Liu, Tao
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[4] Noncanonical amino acids as doubly bio-orthogonal handles for one-pot preparation of protein multiconjugates
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Yeyu Su
Yutong Yu
Xin Wang
Xiaojiang Duan
Haoyu Wang
Xiaomeng Shi
Jing Wang
Xing Yang
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Nature Communications, 14
[5] Site-specific fluorescence labelling of RNA using bio-orthogonal reaction of trans-cyclooctene and tetrazine
Asare-Okai, P. N.
Agustin, E.
Fabris, D.
Royzen, M.
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[6] Site-specific one-pot triple click labeling for DNA and RNA
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Linder, Eva Christina
Becker, Juliane
Jaeschke, Andres
CHEMICAL COMMUNICATIONS, 2018, 54 (83) : 11781 - 11784
[7] A bio-orthogonal functionalization strategy for site-specific coupling of antibodies on vesicle surfaces after self-assembly
Gai, Meiyu
Simon, Johanna
Lieberwirth, Ingo
Mailaender, Volker
Morsbach, Svenja
Landfester, Katharina
POLYMER CHEMISTRY, 2020, 11 (02) : 527 - 540
[8] Targeted Delivery and Site-Specific Activation of β-Cyclodextrin-Conjugated Photosensitizers for Photodynamic Therapy through a Supramolecular Bio-orthogonal Approach
Xue, Evelyn Y.
Shi, Wen-Jing
Fong, Wing-Ping
Ng, Dennis K. P.
JOURNAL OF MEDICINAL CHEMISTRY, 2021, 64 (20) : 15461 - 15476
[9] Site-Specific, Orthogonal Labeling of Proteins in Intact Cells with Two Small Biarsenical Fluorophores
Zuern, Alexander
Klenk, Christoph
Zabel, Ulrike
Reiner, Susanne
Lohse, Martin J.
Hoffmann, Carsten
BIOCONJUGATE CHEMISTRY, 2010, 21 (05) : 853 - 859
[10] Genetically Encoded 1,2-Aminothiols Facilitate Rapid and Site-Specific Protein Labeling via a Bio-orthogonal Cyanobenzothiazole Condensation
Nguyen, Duy P.
Elliott, Thomas
Holt, Matthew
Muir, Tom W.
Chin, Jason W.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 133 (30) : 11418 - 11421

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