Physical mechanism of concentration-dependent fluorescence resonance energy transfer

被引：7
|
作者：
Wang, Jingang [1 ]
Wang, Jiangcai [2 ,3 ]
Mu, Xijiao [2 ]
机构：
[1] Liaoning Shihua Univ, Coll Sci, Computat Ctr Property & Modificat Nanomat, Fushun 113001, Peoples R China
[2] Univ Sci & Technol Beijing, Sch Math & Phys, Beijing Key Lab Magnetophotoelect Composite & Int, Beijing 100083, Peoples R China
[3] Tsinghua Univ, State Key Lab Tribol, Beijing 100084, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2020年 / 231卷
关键词：
Fluorescence resonance energy transfer (FRET); Time-dependent density functional theory (TDDFT); Conformation search; CHARGE-TRANSFER; FRET; EMISSION;
D O I：
10.1016/j.saa.2020.118143
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
We experimentally report fluorescence resonance energy transfer (FRET), using a novel visualization method of excitation-emission mapping. Firstly, both the absorption and fluorescent spectra of donor and acceptor are measured, respectively, under different molecular concentrations for verifying that these two molecules are suitable for exploring FRET. And then, the excitation-emission mappings of FRET are investigated to reveal the internal regular pattern of FRET. Our theoretical calculations strongly support experimental results of FRET. Our experimental results provided a new visualization method to clearly understand the mechanism of FRET. (C) 2020 Published by Elsevier B.V.
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页数：10
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