Study of extraction method of flavonoids from radiated tartary buckwheat mutants

Experiment was made on the method, condition of extracting flavonoids from three varities of tartary buckwheat, which had received radiation. On the basis of single factor, such as ethanol concentration, feed liquid, extracting temperature and time, 4 factor 3 level orthogonal test were designed. The results indicated that: the order of each factor is extracting emperature > ethanol solution percentage(A) > during of extraction (D) > feed liquid ratio (B); the most optimum condition is the combination of A(2)B(2)C(3)D, i.e. ethanol 60%, feed liquid ratio 1:20, extracting temperature 70 degrees C, and extracting duration 90 minutes; the role of pretreatment of material in improving the efficiency of extracting flavone was also discussed.