A novel DNA hairpin substrate for bleomycin

被引：16

作者：

Akiyama, Yoshitsugu ^{[1
]}

Ma, Qian ^{[1
]}

Edgar, Erin ^{[2
]}

Laikhter, Andrei ^{[2
]}

Hecht, Sidney M. ^{[1
]}

机构：

[1] Univ Virginia, Dept Biol & Chem, Charlottesville, VA 22904 USA

[2] Integrated DNA Technologies, Coralville, IA 52241 USA

来源：

ORGANIC LETTERS | 2008年 / 10卷 / 11期

关键词：

D O I：

10.1021/ol800445x

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A 16-nucleotide DNA hairpin containing 4-aminobenzo[g]quinazoline-2-one 2'-deoxyribose at position 15 has been prepared and found to lack significant fluorescence, When treated with Fe(II).BLM, the hairpin was found to undergo oxidative transformation selectively at position 15. The predominant fluorescent product was characterized and quantified. The pro-fluorescent DNA hairpin was used as a substrate for 15 bleomycin congeners, and the results were compared with those obtained following cleavage of a radiolabeled DNA duplex and PAGE analysis.

引用

页码：2127 / 2130

页数：4

共 50 条

[21] Novel amplification of DNA in a hairpin structure: towards a radical elimination of PCR errors from amplified DNA
Kaur, M
Makrigiorgos, GM
NUCLEIC ACIDS RESEARCH, 2003, 31 (06)
[22] SUBSTRATE-SPECIFICITY OF BLEOMYCIN HYDROLASE
SEBTI, SM
DELEON, JC
MA, LT
HECHT, SM
LAZO, JS
BIOCHEMICAL PHARMACOLOGY, 1989, 38 (01) : 141 - 147
[23] Model for DNA hairpin denaturation
S. Cuesta-López
M. Peyrard
D. J. Graham
The European Physical Journal E, 2005, 16 : 235 - 246
[24] Model for DNA hairpin denaturation
Cuesta-López, S
Peyrard, M
Graham, DJ
EUROPEAN PHYSICAL JOURNAL E, 2005, 16 (03): : 235 - 246
[25] Hairpin structures in DNA words
Kari, Lila
Konstantinidis, Stavros
Losseva, Elena
Sosik, Petr
Thierrin, Gabriel
DNA COMPUTING, 2006, 3892 : 158 - 170
[26] Bleomycin-medicated degradation of aristeromycin-containing DNA. Novel dehydrogenation activity of ironII-bleomycin
1600, (113):
[27] DNA CLEAVAGE ACTIVITY OF LIBLOMYCIN (NK313), A NOVEL ANALOG OF BLEOMYCIN
KURAMOCHI, H
MOTEGI, A
TAKAHASHI, K
TAKEUCHI, T
JOURNAL OF ANTIBIOTICS, 1988, 41 (12): : 1846 - 1853
[28] NOVEL DNA PHOTOCLEAVING AGENTS WITH HIGH DNA-SEQUENCE SPECIFICITY RELATED TO THE ANTIBIOTIC BLEOMYCIN A(2)
KURODA, R
SATOH, H
SHINOMIYA, M
WATANABE, T
OTSUKA, M
NUCLEIC ACIDS RESEARCH, 1995, 23 (09) : 1524 - 1530
[29] A novel uracil-DNA glycosylase family related to the helix-hairpin-helix DNA glycosylase superfamily
Chung, JH
Im, EK
Park, HY
Kwon, JH
Lee, S
Oh, J
Hwang, KC
Lee, JH
Jang, Y
NUCLEIC ACIDS RESEARCH, 2003, 31 (08) : 2045 - 2055
[30] FUNCTIONAL ANALOGS OF BLEOMYCIN - DNA CLEAVAGE BY BLEOMYCIN AND HEMIN - INTERCALATORS
HASHIMOTO, Y
IIJIMA, H
NOZAKI, Y
SHUDO, K
BIOCHEMISTRY, 1986, 25 (18) : 5103 - 5110

← 1 2 3 4 5 →