Autophosphorylation of archaeal Cdc6 homologues is regulated by DNA

被引：41
|
作者：
Grabowski, B [1 ]
Kelman, Z [1 ]
机构：
[1] Univ Maryland, Maryland Biotechnol Inst, Ctr Adv Res Biotechnol, Rockville, MD 20850 USA
来源：
JOURNAL OF BACTERIOLOGY | 2001年 / 183卷 / 18期
关键词：
D O I：
10.1128/JB.183.18.5459-5464.2001
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The initiator protein Cdc6 (Cdc18 in fission yeast) plays an essential role in the initiation of eukaryotic DNA replication. In yeast the protein is expressed before initiation of DNA replication and is thought to be essential for loading of the helicase onto origin DNA. The biochemical properties of the protein, however, are largely unknown. Using three archaeal homologues of Cdc6, it was found that the proteins are autophosphorylated on Ser residues. The winged-helix domain at the C terminus of Cdc6 interacts with DNA, which apparently regulates the autophosphorylation reaction. Yeast Cdc18 was also found to autophosphorylate, suggesting that this function of Cdc6 may play a widely conserved and essential role in replication initiation.
引用
收藏
页码：5459 / 5464
页数：6
相关论文
共 50 条
  • [21] The role and regulation of the preRC component Cdc6 in the initiation of premeiotic DNA replication
    Ofir, Y
    Sagee, S
    Guttmann-Raviv, N
    Pnueli, L
    Kassir, Y
    MOLECULAR BIOLOGY OF THE CELL, 2004, 15 (05) : 2230 - 2242
  • [22] ATPase-dependent cooperative binding of ORC and Cdc6 to origin DNA
    Christian Speck
    Zhiqiang Chen
    Huilin Li
    Bruce Stillman
    Nature Structural & Molecular Biology, 2005, 12 : 965 - 971
  • [23] ATPase-dependent cooperative binding of ORC and Cdc6 to origin DNA
    Speck, C
    Chen, ZQ
    Li, HL
    Stillman, B
    NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2005, 12 (11) : 965 - 971
  • [24] Identification and characterization of Saccharomyces cerevisiae Cdc6 DNA-binding properties
    Feng, L
    Wang, B
    Driscoll, B
    Jong, A
    MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (05) : 1673 - 1685
  • [25] The Schizosaccharomyces pombe cdc6 gene encodes the catalytic subunit of DNA polymerase δ
    Yuichi Iino
    Masayuki Yamamoto
    Molecular and General Genetics MGG, 1997, 254 : 93 - 97
  • [26] The regulated association of Cdt1 with minichromosome maintenance proteins and Cdc6 in mammalian cells
    Cook, JG
    Chasse, DAD
    Nevins, JR
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (10) : 9625 - 9633
  • [27] Cell cycle regulated expression of mammalian CDC6 is dependent on E2F
    Hateboer, G
    Wobst, A
    Petersen, BO
    Le Cam, L
    Vigo, E
    Sardet, C
    Helin, K
    MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (11) : 6679 - 6697
  • [28] CDC6 expression is regulated by lineage-specific transcription factor GATA1
    Fernandez-Morales, Barbara
    Pavon, Leticia
    Cales, Carmela
    CELL CYCLE, 2012, 11 (16) : 3055 - 3066
  • [29] CDC6 is a possible biomarker for hepatocellular carcinoma
    Kong, De-Gang
    Yao, Fu-Zhou
    INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 2021, 14 (07): : 811 - 818
  • [30] Human and xenopus proteins related to the yeast CDC6/CDC18+ regulators of DNA replication.
    Williams, RS
    Stillman, B
    JOURNAL OF INVESTIGATIVE MEDICINE, 1996, 44 (03) : A198 - A198
12345