Research of Purification and Antioxidant Function of the Chondroitin Sulfate Extracted from Sturgeon

Sturgeon cartilage from Siberian was experimented as raw material and the purification and antioxidant ability of chondroitin sulfate were studied in this paper. The best condition of purification and separation was determined. The chondroitin sulfate was obtained by Ion-exchange Column of DEAE - Sepharose FF and final purification of chondroitin sulfate was obtained. Three products marked as CS-1, CS-2 and CS-3 were obtained according to the chromatographic peak and the white purifications were the same as the standard substances; Furthermore, the antioxidant ability of chondroitin sulfate was determined by using the method of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay, hydroxyl radical (OH.) scavenging capacity assay and the reducing power assay. The results showed that the DPPH scavenging ability, OH. Scavenging ability and the reducing power of the chondroitin sulfate were well correlated with its increasing concentrations.