Molecular Changes in Corn Stover Lignin Resulting from Pretreatment Chemistry

被引:8
|
作者
Min, Douyong [1 ,2 ]
Wang, Shuang-fei [1 ,2 ]
Chang, Hou-min [3 ]
Jameel, Hasan [3 ]
Lucia, Lucian [3 ,4 ]
机构
[1] Guangxi Univ, Dept Paper Sci & Technol, Nanning 530004, Guangxi, Peoples R China
[2] Guangxi Univ, Prov Key Lab Clean Pulping & Pollut Control, Nanning 530004, Guangxi, Peoples R China
[3] North Carolina State Univ, Dept Forest & Biomat, Raleigh, NC 27695 USA
[4] North Carolina State Univ, Dept Chem, Raleigh, NC 27695 USA
来源
BIORESOURCES | 2017年 / 12卷 / 03期
关键词
Corn stover; Pretreatments; Cellulolytic Enzyme Lignin; Structure; H-1-C-13 HSQC NMR; ENZYMATIC-HYDROLYSIS; NMR-SPECTROSCOPY; CATALPA SAWDUST; DFRC METHOD; SACCHARIFICATION; DELIGNIFICATION; FRACTIONATION; CLEAVAGE; STEMS; MODEL;
D O I
10.15376/biores.12.3.6262-6275
中图分类号
TB3 [工程材料学]; TS [轻工业、手工业、生活服务业];
学科分类号
0805 ; 080502 ; 0822 ;
摘要
Lignin is an amorphous polymer that limits the enzymatic conversion of polysaccharides to fermentable sugars. Thus, a pretreatment that can enhance the accessibility of carbohydrates is a key step of successful biofuel conversion schemes. In this study, corn stover was fractioned into stem, cob, and leaf because their lignin is different. To elucidate the lignin changes, autohydrolysis, diluted acid, and alkali pretreatments were applied on the samples, followed by the isolation of cellulolytic enzyme lignin preparations. Alkaline nitrobenzene oxidation, C-13-Nuclear Magnetic Resonance (NMR), and H-1-C-13 heteronuclear single quantum coherence NMR were used to profile the lignin changes. The results indicated that corn stover lignin is a p-hydroxyphenyl-guaiacyl-syringyl-type lignin that incorporates p-coumarate and ferulate esters. The beta-aryl-ether was the most abundant inter-unit linkage, followed by condensed linkages, e.g. pino-/syringaresinol, phenylcoumaran, and spirodienone. As for the non-pretreated samples, leaf lignin was more condensed than stem lignin and cob lignin. More lignin was removed by the alkali pretreatment due to more cleavage of beta-aryl-ether linkages. As a comparison, more condensed linkages were generated by the acidic pretreatments. The decrease of the syringyl/guaiacyl ratio indicated that the residual lignin became more condensed and confirmed that guaiacyl and p-hydroxyphenyl units were more stable than syringyl units during the pretreatment.
引用
收藏
页码:6262 / 6275
页数:14
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