Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover

被引：81
|
作者：
Su, Yingjie [1 ]
Yu, Xiaoxiao [1 ]
Sun, Yang [1 ]
Wang, Gang [1 ]
Chen, Huan [1 ]
Chen, Guang [1 ]
机构：
[1] Jilin Agr Univ, Coll Life Sci, Changchun 130118, Jilin, Peoples R China
来源：
SCIENTIFIC REPORTS | 2018年 / 8卷
关键词：
SOLID-STATE FERMENTATION; EXPLODED WHEAT-STRAW; PHANEROCHAETE-CHRYSOSPORIUM; ENZYMATIC-HYDROLYSIS; LACCASE DETOXIFICATION; MANGANESE PEROXIDASE; ETHANOL-PRODUCTION; SUGARCANE BAGASSE; TRAMETES-HIRSUTA; HOT-WATER;
D O I：
10.1038/s41598-018-23626-6
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The biological pretreatment of lignocellulosic biomass is a low-cost and eco-friendly method for facilitating enzymatic hydrolysis. In this study, strains with lignin depletion capability were screened using a high-throughput screening method. Sixty-three strains were screened out and Myrothecium verrucaria secreted three lignin-degrading enzymes simultaneously during the bio-pretreatment process. The activity levels of laccase, lignin peroxidase and manganese peroxidase were 6.61, 0.78 and 1.31 U g(-1) dry biomass. The content of lignin in corn stover decreased by 42.30% after biopretreatment, and the conversion rate increased by 123.84% during the subsequent saccharification process in comparison with the untreated corn stover. Furthermore, the effects of bio-pretreatment on the structure of corn stover were presented using a scanning electron microscope (SEM), Brunauer-Emmet-Teller (BET), X-ray diffractometer (XRD) and Fourier transform infrared spectroscopy (FTIR). The results showed that M. V. is a promising lignin-degrading fungus. This research demonstrated an efficient pretreatment approach for enhancing the enzymatic saccharification of corn stover.
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页数：11
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