Cellular Processes Involved in Jurkat Cells Exposed to Nanosecond Pulsed Electric Field

被引:9
|
作者
Li, Huijuan [1 ]
Liu, Shibin [1 ]
Yang, Xue [1 ]
Du, Yongqian [1 ]
Luo, Jiezhang [1 ]
Tan, Jie [1 ]
Sun, Yulong [2 ]
机构
[1] Northwestern Polytech Univ, Sch Elect & Informat, Xian 710072, Shaanxi, Peoples R China
[2] Northwestern Polytech Univ, Key Lab Space Biosci & Biotechnol, Sch Life Sci, Xian 710072, Shaanxi, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
nanosecond pulsed electric field (nsPEF); Jurkat; microarray; bioinformatics analysis; molecular dynamics simulation; MESSENGER-RNA; LEUKEMIA; EXPRESSION; REGULATOR; PLATFORM; PTEN;
D O I
10.3390/ijms20235847
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recently, nanosecond pulsed electric field (nsPEF) has been considered as a new tool for tumor therapy, but its molecular mechanism of function remains to be fully elucidated. Here, we explored the cellular processes of Jurkat cells exposed to nanosecond pulsed electric field. Differentially expressed genes (DEGs) were acquired from the GEO2R, followed by analysis with a series of bioinformatics tools. Subsequently, 3D protein models of hub genes were modeled by Modeller 9.21 and Rosetta 3.9. Then, a 100 ns molecular dynamics simulation for each hub protein was performed with GROMACS 2018.2. Finally, three kinds of nsPEF voltages (0.01, 0.05, and 0.5 mV/mm) were used to simulate the molecular dynamics of hub proteins for 100 ns. A total of 1769 DEGs and eight hub genes were obtained. Molecular dynamic analysis, including root mean square deviation (RMSD), root mean square fluctuation (RMSF), and the Rg, demonstrated that the 3D structure of hub proteins was built, and the structural characteristics of hub proteins under different nsPEFs were acquired. In conclusion, we explored the effect of nsPEF on Jurkat cell signaling pathway from the perspective of molecular informatics, which will be helpful in understanding the complex effects of nsPEF on acute T-cell leukemia Jurkat cells.
引用
收藏
页数:45
相关论文
共 50 条
  • [31] Nanopore Formation in Cells Exposed to Nanosecond Electric Pulses
    Pakhomov, Andrei G.
    Bowman, Angela M.
    Ibey, Bennett L.
    Pakhomova, Olga N.
    BIOPHYSICAL JOURNAL, 2010, 98 (03) : 375A - 375A
  • [32] Nanosecond pulsed electric fields: microdosimetry on single cells
    Merla, Caterina
    Paffi, Alessandra
    Liberti, Micaela
    Apollonio, Francesca
    Danei, Federico
    Leveque, Philippe
    d'Inzeo, Guglielmo
    PROCEEDINGS OF THE FOURTH EUROPEAN CONFERENCE ON ANTENNAS AND PROPAGATION, 2010,
  • [33] Nanosecond pulsed electric field generators for the study of subcellular effects
    Kolb, JF
    Kono, S
    Schoenbach, KH
    BIOELECTROMAGNETICS, 2006, 27 (03) : 172 - 187
  • [34] Microchamber Setup Characterization for Nanosecond Pulsed Electric Field Exposure
    Arnaud-Cormos, Delia
    Leveque, Philippe
    Wu, Yu-Hsuan
    Sanders, Jason M.
    Gundersen, Martin A.
    Vernier, P. Thomas
    IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 2011, 58 (06) : 1656 - 1662
  • [35] Nanosecond pulsed electric field induces calcium mobilization in osteoblasts
    Zhou, Ping
    He, Fei
    Han, Yu
    Liu, Bin
    Wei, Shicheng
    BIOELECTROCHEMISTRY, 2018, 124 : 7 - 12
  • [36] Excitation of murine cardiac myocytes by nanosecond pulsed electric field
    Azarov, Jan E.
    Semenov, Iurii
    Casciola, Maura
    Pakhomov, Andrei G.
    JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY, 2019, 30 (03) : 392 - 401
  • [37] Effects of Nanosecond Pulsed Electrical Fields (nsPEFs) on the Cell Cycle of CHO and Jurkat Cells
    Mahlke, Megan A.
    Navara, Christopher
    Ibey, Bennett L.
    OPTICAL INTERACTIONS WITH TISSUE AND CELLS XXV; AND TERAHERTZ FOR BIOMEDICAL APPLICATIONS, 2014, 8941
  • [38] Effects of Nanosecond Pulsed Electric Field Exposure on Arabidopsis thaliana
    Eing, Christian J.
    Bonnet, Simone
    Pacher, Michael
    Puchta, Holger
    Frey, Wolfgang
    IEEE TRANSACTIONS ON DIELECTRICS AND ELECTRICAL INSULATION, 2009, 16 (05) : 1322 - 1328
  • [39] Activation of autophagy in response to nanosecond pulsed electric field exposure
    Ullery, Jody C.
    Tarango, Melissa
    Roth, Caleb C.
    Ibey, Bennett L.
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2015, 458 (02) : 411 - 417
  • [40] Oxidative effects of nanosecond pulsed electric field exposure in cells and cell-free media
    Pakhomova, Olga N.
    Khorokhorina, Vera A.
    Bowman, Angela M.
    Rodaite-Riseviciene, Raminta
    Saulis, Gintautas
    Xiao, Shu
    Pakhomov, Andrei G.
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2012, 527 (01) : 55 - 64