Toxic effects of the Emamectin Benzoate exposure on cultured human bronchial epithelial (16HBE) cells

被引：25
|
作者：
Niu, Chenguang [1 ]
Wang, Chunli [2 ]
Wu, Guangyao [3 ]
Yang, Jingnan [3 ]
Wen, Yanan [4 ]
Meng, Shuangshuang [4 ]
Lin, Xuhong [4 ]
Pang, Xiaobin [2 ]
An, Lei [3 ,5 ]
机构：
[1] Henan Univ, Affiliated Hosp 1, Key Lab Clin Resources Translat, Kaifeng 475000, Henan, Peoples R China
[2] Henan Univ, Sch Pharm, Pharmaceut Inst, Kaifeng 475000, Henan, Peoples R China
[3] Henan Univ, Huaihe Hosp, Translat Med Ctr, Kaifeng 475000, Henan, Peoples R China
[4] Henan Univ, Huaihe Hosp, Dept Clin Lab, Kaifeng 475000, Henan, Peoples R China
[5] Henan Univ, Henan Med Sch, Inst Biomed Sci, Kaifeng 475000, Henan, Peoples R China
来源：
ENVIRONMENTAL POLLUTION | 2020年 / 257卷
基金：
中国国家自然科学基金;
关键词：
Pesticides exposure; Emamectin benzoate; Cytotoxicity; Human bronchial epithelial (16HBE) cells; Mitochondrial apoptosis; DNA-DAMAGE; APOPTOSIS; MITOCHONDRIA; PESTICIDES; CASPASES; REPAIR;
D O I：
10.1016/j.envpol.2019.113618
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Pesticides pollution has caused serious environmental problems in recent years, and mounting evidence has shown that more and more insecticides have serious risk in human health. Emamectin Benzoate formally regarded as a highly safety insecticide based on its exclusive targets, but the cytotoxicity to human lung was ignored for a long time. In the present study, bioassay experiments were used to assess the toxicity of the Emamectin Benzoatein on human non-target cells including cell viability assay, DNA damage assay, flow cytometer assay and western blotting assay. The results indicated that Emamectin Benzoatecan cause the inhibition of the proliferation, cytochrome c release, activation of caspase-3/9 and increase Bax/Bcl-2 ratio, which means it induced the cytotoxicity on 16HBE cells associated with the mitochondrial apoptosis. Besides, the DNA damge caused by the Emamectin Benzoate suggest it has a potential genotoxic effect on human lung cells. (C) 2019 Elsevier Ltd. All rights reserved.
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页数：7
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