Correlated cleavage of single- and double-stranded substrates by uracil-DNA glycosylase

被引：22

作者：

Sidorenko, Viktoriya S. ^{[1
]}

Mechetin, Grigory V. ^{[1
]}

Nevinsky, Georgy A. ^{[1
]}

Zharkov, Dmitry O. ^{[1
]}

机构：

[1] SB RAS Inst Chem Biol & Fundamental Med, Novosibirsk 630090, Russia

来源：

FEBS LETTERS | 2008年 / 582卷 / 03期

基金：

俄罗斯基础研究基金会;

关键词：

DNA repair; base excision; processivity; correlated cleavage; uracil-DNA glycosylase;

D O I：

10.1016/j.febslet.2008.01.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Uracil-DNA glycosylase (Ung) can quickly locate uracil bases in an excess of undamaged DNA. DNA glycosylases may use diffusion along DNA to facilitate lesion search, resulting in processivity, the ability of glycosylases to excise closely spaced lesions without dissociating from DNA. We propose a new assay for correlated cleavage and analyze the processivity of Ung. Ung conducted correlated cleavage on double- and single-stranded substrates; the correlation declined with increasing salt concentration. Proteins in cell extracts also decreased Ung processivity. The correlated cleavage was reduced by nicks in DNA, suggesting the intact phosphodiester backbone is important for Ung processivity. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：410 / 414

页数：5

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