Uncovering mechanisms of RT-LAMP colorimetric SARS-CoV-2 detection to improve assay reliability

被引：7
|
作者：
Suarez, Gianmarco D. [1 ,7 ]
Suarez, Domenick A. [7 ]
Tang, Yuki Yu Kiu [7 ]
Zhang, Jing-Xuan [4 ]
Li, Jun [4 ,5 ,6 ]
Nagl, Stefan [1 ]
Cheung, Peter Pak-Hang [2 ,3 ,7 ]
机构：
[1] Hong Kong Univ Sci & Technol, Dept Chem, Clear Water Bay, Kowloon, Hong Kong, Peoples R China
[2] Chinese Univ Hong Kong, Prince Wales Hosp, Fac Med, Dept Chem Pathol,Shatin, Hong Kong, Peoples R China
[3] Chinese Univ Hong Kong, Prince Wales Hosp, Li Ka Shing Inst Hlth Sci, Shatin, Li Ka Shing Med Sci Bldg, Hong Kong, Peoples R China
[4] Southern Univ Sci & Technol, Dept Chem, Shenzhen 518055, Guangdong, Peoples R China
[5] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China
[6] Tsinghua Univ, Key Lab Organ Optoelect & Mol Engn, Minist Educ, Beijing 100084, Peoples R China
[7] Quommni Technol Ltd, Hong Kong, Peoples R China
来源：
ANALYTICAL METHODS | 2022年 / 14卷 / 04期
关键词：
MEDIATED ISOTHERMAL AMPLIFICATION;
D O I：
10.1039/d1ay01395e
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Improved diagnostics are needed to manage the ongoing COVID-19 pandemic. In this study, we enhanced the color changes and sensitivity of colorimetric SARS-CoV-2 RT-LAMP assays based on triarylmethane dyes. We determined a mechanism for the color changes and obtained sensitivities of 10 RNA copies per microliter.
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页码：378 / 382
页数：5
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