In vitro culture of zygotic embryos of Ilex species

被引：18

作者：

Sansberro, PA ^{[1
]}

Rey, HY ^{[1
]}

Mroginski, LA ^{[1
]}

机构：

[1] Inst Bot Nordeste, Fac Ciencias Agr, RA-3400 Corrientes, Argentina

来源：

HORTSCIENCE | 2001年 / 36卷 / 02期

关键词：

plant regeneration; germination; embryo culture; holly; propagation;

D O I：

10.21273/HORTSCI.36.2.351

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Plants of Ilex argentina L., I. brasiliensis (S.) L., I. brevicuspis R., I. dumosa R., I. integerrima (V.C.) L., I. microdonta R., I. pseudoboxus R., and I. theezans C.M. were obtained by immature embryo culture. Heart-stage zygotic embryos were removed from immature fruits and cultured aseptically on quarter-strength Murashige and Skoog medium,vith 3% sucrose, 0.65% agar, and 0.1 mg.L-1 zeatin. Cultures were incubated at 27 +/- 2 degreesC for 4 weeks, in darkness and subsequently transferred to a culture roam with a 14-hour photoperiod (116 mu mol.m(-2).s(-1)) for another 4 weeks. Seedlings with two leaves, derived from germinated embryos, were successfully transplanted to pots containing 1 peat: 1 perlite : 1 sand (v/v) and were maintained in greenhouse conditions. From 95% to 100% of transplanted seedlings survived. Chemical name used: 6-(4-hydroxy-3-methylbut-2-enylamino) purine (zeatin).

引用

页码：351 / 352

页数：2

共 50 条

[21] In vitro germination of 'Murmuru' zygotic embryos (Astrocaryum ulei)
Scherwinski Pereira, Jonny Everson
Silva Maciel, Tissiane Maria
da Silva Costa, Frederico Henrique
Alves Pereira, Maria Aparecida
[J]. CIENCIA E AGROTECNOLOGIA, 2006, 30 (02): : 251 - 256
[22] Application of analogue brassinosteroide (Biobras 16®) germination and growth in vitro culture of zygotic embryos and acclimatization of bocaiuva
Borcioni, Elis
Bonato Negrelle, Raquel Rejane
[J]. CIENCIA RURAL, 2012, 42 (02): : 270 - 275
[23] STORAGE LIPID-ACCUMULATION BY ZYGOTIC AND SOMATIC EMBRYOS IN CULTURE
AVJIOGLU, A
KNOX, RB
[J]. ANNALS OF BOTANY, 1989, 63 (04) : 409 - 420
[24] In vitro response of sunflower immature zygotic embryos to ethylene inhibitors
Tuleja, Monika
Zeren, Yasemin
Ozad, Azer
Konieczny, Robert
Plachno, Bartosz J.
[J]. ACTA BIOLOGICA CRACOVIENSIA SERIES BOTANICA, 2010, 52 : 94 - 94
[25] In vitro morphogenic response from zygotic embryos of Genipa Americana
Araujo de Oliveira, Annie Carolina
Machado, Caroline de Araujo
Resende de Oliveira, Leila Albuquerque
Cruz da Silva, Ana Veruska
Ledo, Ana da Silva
[J]. CIENCIA RURAL, 2017, 47 (10):
[26] Physiological development of zygotic embryos of heliconias propagated in vitro and conventionally
Ulisses, Claudia
Morais, Marciana
Barbosa, Marta R.
Albuquerque, Cynthia C.
Willadino, Lilia
Camara, Terezinha R.
[J]. HORTICULTURA BRASILEIRA, 2018, 36 (02) : 229 - 234
[27] In vitro germination of babassu: influence of growth regulators in zygotic embryos
Leite, Mariluza Silva
Silva, Fabiano Guimaraes
Pereira, Flavia Dionisio
Alberto, Paula Sperotto
Boffino de Almeida Monteiro-Hara, Alessandra Cristina
[J]. ACTA SCIENTIARUM-AGRONOMY, 2014, 36 (04): : 449 - 456
[28] Induction and proliferation of axillary shoots from in vitro culture of Paeonia lactiflora Pall. mature zygotic embryos
Shen, Miaomiao
Tang, Zhengjiao
Teixeira da Silva, Jaime A.
Yu, XiaoNan
[J]. NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE, 2015, 43 (01) : 42 - 52
[29] In vitro regeneration of plantlets from immature zygotic embryos of Picea chihuahuana Martinez, an endemic Mexican endangered species
Rosas, MM
Avila, VMC
Boettler, RB
[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2001, 37 (01) : 73 - 78
[30] Manifestation of embryogenic potential in culture of zygotic embryos of Quercus robur L
Ostrolucka, MG
Krajmerova, D
[J]. ACTA SOCIETATIS BOTANICORUM POLONIAE, 1996, 65 (1-2) : 37 - 41

← 1 2 3 4 5 →