Size-Tunable Organic Nanodot Arrays: A Versatile Platform for Manipulating and Imaging Cells

被引：17

作者：

Pi, Fuwei ^{[1
]}

Dillard, Pierre ^{[1
,2
]}

Alameddine, Ranime ^{[1
]}

Benard, Emmanuelle ^{[1
]}

Wahl, Astrid ^{[1
]}

Ozerov, Igor ^{[1
]}

Charrier, Anne ^{[1
]}

Limozin, Laurent ^{[2
]}

Sengupta, Kheya ^{[1
]}

机构：

[1] Aix Marseille Univ, CNRS, CINaM UMR 7325, F-13288 Marseille, France

[2] Aix Marseille Univ, Inserm U1067, CNRS UMR7333, Lab Adhes & Inflammat, F-13288 Marseille, France

来源：

NANO LETTERS | 2015年 / 15卷 / 08期

基金：

欧洲研究理事会;

关键词：

Nanobiopatterning; antibody nanodots; nanosphere lithography; T lymphocyte; SUPPORTED LIPID-BILAYERS; T-CELLS; IMMUNOLOGICAL SYNAPSE; NANOSPHERE LITHOGRAPHY; COLLOIDAL LITHOGRAPHY; CELLULAR ADHESION; NANOSCALE CONTROL; FOCAL ADHESIONS; SURFACE; ACTIVATION;

D O I：

10.1021/acs.nanolett.5b01400

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Arrays of protein nanodots with dot-size tuned independently of spacing (e.g., similar to 400 to 600 nm diameter for 900 nm spacing) are fabricated. The mechanism of size control is demonstrated, by numerical simulations, to arise from shadow effects during deposition of a sacrificial metal mask. We functionalize the nanodots with antibodies and embed them in a polymer-cushion or in lipid-bilayers or transfer them to soft elastomers. Their ability to influence cell architecture and local membrane organization is demonstrated in T-lymphocytes, using reflection interference contrast and total internal reflection fluorescence microscopy.

引用

页码：5178 / 5184

页数：7

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