Genetic diversity and population structure of mahaleb cherry (Prunus mahaleb L.) and sweet cherry (Prunus avium L.) using SRAP markers

被引:30
|
作者
Abedian, Masoud [1 ]
Talebi, Majid [1 ]
Golmohammdi, Hamid Reza [1 ]
Sayed-Tabatabaei, Badraldin Ebrahim [1 ]
机构
[1] Isfahan Univ Technol, Coll Agr, Dept Agr Biotechnol, Esfahan 8415683111, Iran
关键词
Mahaleb cherry; Sweet cherry; Genetic diversity; SRAP; AMOVA; MOLECULAR CHARACTERIZATION; DNA;
D O I
10.1016/j.bse.2011.10.005
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genetic diversity of 47 Mahaleb cherry genotypes (Prunus mahaleb L.) and six sweet cherry accessions (Prunus avium L) was studied using 13 SRAP primer combinations. The number of polymorphic fragments detected per primer combination ranged from 1 to 10 bands with an average 4.23 bands. Average PIC was 0.42 over all primer combinations. Cluster analysis using UPGMA method and Dice's coefficient grouped the species into two main clusters with similarity coefficient ranging from 0.16 to 0.93. Sum of first three PCAs could be represented most of (65.64%) the total variation in the original dimensions and confirmed the results of cluster analysis. Based on AMOVA results, the allele numbers among groups and species studied in this research were more than those observed within them. This is the first report of using SRAP marker as a tool for determining genetic variation among and within P. mahaleb and P. avium. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:112 / 117
页数:6
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