Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

被引：31
|
作者：
da Silva, Luiz Claudio [1 ]
Garcia, Thiago [1 ]
Mori, Michela [2 ]
Sandri, Giuseppina [2 ]
Bonferoni, Maria Cristina [2 ]
Finotelli, Priscila V. [1 ]
Cinelli, Leonardo P. [1 ]
Caramella, Carla [2 ]
Cabral, Lucio M. [1 ]
机构：
[1] Univ Fed Rio de Janeiro, Drug Dept, BR-21941902 Rio De Janeiro, RJ, Brazil
[2] Univ Pavia, Dept Drug Sci, I-27100 Pavia, Italy
来源：
INTERNATIONAL JOURNAL OF NANOMEDICINE | 2012年 / 7卷
关键词：
nanoparticles; chitosan; chondroitin sulfate; fucoidan; aPTT; Caco-2; CHITOSAN-CHONDROITIN SULFATE; DRUG-DELIVERY; CAPILLARY-ELECTROPHORESIS; CONTROLLED-RELEASE; SYSTEMS; COMPLEX; FUCAN; DOXORUBICIN; ALGINATE; CARRIERS;
D O I：
10.2147/IJN.S31632
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
The aim of this study was to produce and characterize nanoparticles (NPs), combining chondroitin sulfate (CS) and fucoidan (FC) with chitosan for therapeutic purposes. These NPs were characterized by dynamic light scattering, zeta potential determination, and transmission electronic microscopy. The anticoagulant activity was determined for FC NPs and compared with FC solution at the same concentration. FC NPs showed regular shapes and better anticoagulant activity than free polysaccharide solution. FC solution did not affect coagulation compared to FC NPs, which increased up to two-fold, even at a lower concentration. Cytotoxicity and permeability tests were conducted using Caco-2 cell monolayer, exhibiting no toxic effect in this cell line and higher permeability for NP2 samples than FC solution at the same concentration.
引用
收藏
页码：2975 / 2986
页数：12
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