Kidney injury molecule-1 staining in renal allograft biopsies 10 days after transplantation is inversely correlated with functioning proximal tubular epithelial cells

被引：19

作者：

Bank, Jonna R. ^{[1
]}

van der Pol, Pieter ^{[1
]}

Vreeken, Dianne ^{[1
]}

Monge-Chaubo, Catherine ^{[1
]}

Bajema, Ingeborg M. ^{[2
]}

Schlagwein, Nicole ^{[1
]}

van Gijlswijk, Danielle J. ^{[1
]}

van der Kooij, Sandra W. ^{[1
]}

Reinders, Marlies E. J. ^{[1
]}

de Fijter, Johan W. ^{[1
]}

van Kooten, Cees ^{[1
]}

机构：

[1] Leiden Univ, Med Ctr, Dept Internal Med Nephrol, Leiden, Netherlands

[2] Leiden Univ, Med Ctr, Dept Pathol, Leiden, Netherlands

来源：

NEPHROLOGY DIALYSIS TRANSPLANTATION | 2017年 / 32卷 / 12期

关键词：

delayed graft function; kidney transplant recipients; KIM-1 and NGAL staining; proximal tubular epithelial cells; renal allograft biopsies; DELAYED GRAFT FUNCTION; GELATINASE-ASSOCIATED LIPOCALIN; URINARY; KIM-1; BIOMARKER; ISCHEMIA; SURVIVAL; NGAL; METAANALYSIS; EXPRESSION;

D O I：

10.1093/ndt/gfx286

中图分类号：

R3 [基础医学]; R4 [临床医学];

学科分类号：

1001 ; 1002 ; 100602 ;

摘要：

Background. Kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) are promising bio-markers for monitoring delayed graft function (DGF) after kidney transplantation. Here we investigated localization and distribution of KIM-1 and NGAL staining in renal allograft biopsies and studied their association with histological features, functional DGF (fDGF) and the tubular function slope (TFS), a functioning proximal tubular epithelial cell (PTEC) marker. Methods. Day 10 protocol biopsies of 64 donation after circulatory death recipients were stained for KIM-1 and NGAL and the positive area was quantified using ImageJ software. Biopsies were scored according to Banff and acute tubular necrosis (ATN) criteria. A (99m)technetium-mercaptoacetyltriglycine (Tc-99m-MAG3)-renography was performed to calculate TFS. Results. KIM-1 staining was located on the brush border of tubular epithelial cells (TECs) and correlated with denudation, while NGAL was present more focally in a cytoplasmic distribution. KIM-1 and NGAL staining were not correlated and no co-localization was observed. Quantitative stainings were not associated with fDGF, but KIM-1 tended to be higher in patients with prolonged fDGF (>= 21 days; P = 0.062). No correlation was observed between the quantitative tissue stainings and urinary KIM-1 or NGAL. Quantitative KIM-1 staining was inversely correlated with the TFS (Spearman's rho = -0.53; P < 0.001), whereas NGAL was not. The latter finding might be because cortical NGAL staining is dependent on filtration and subsequent reabsorption by functioning PTECs. Staining of NGAL was indeed restricted to PTECs, as shown by co-localization with a PTEC-specific lectin. Conclusions. KIM-1 and NGAL staining showed different localization and distribution. Quantitative KIM-1 staining was inversely correlated with functioning PTECs.

引用

页码：2132 / 2141

页数：10

共 23 条

[11] Low Ratio of CD68/Kidney Injury Molecule-1 (KIM-1) Expression in the Proximal Tubules of Renal Biopsies Is Associated with Worse Renal Recovery at 6 Month Follow-Up
Ward, Nicholas D.
Zhang, Ping L.
LABORATORY INVESTIGATION, 2017, 97 : 411A - 411A
[12] Low Ratio of CD68/Kidney Injury Molecule-1 (KIM-1) Expression in the Proximal Tubules of Renal Biopsies Is Associated with Worse Renal Recovery at 6 Month Follow-Up
Ward, Nicholas D.
Zhang, Ping L.
MODERN PATHOLOGY, 2017, 30 : 411A - 411A
[13] KIDNEY INJURY MOLECULE-1 AND HEART-TYPE FATTY ACID BINDING PROTEIN AS NOVEL EARLY MARKERS OF PROXIMAL AND DISTAL RENAL TUBULAR DYSFUNCTION IN CHILDREN WITH β-THALASSAEMIA MAJOR
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HAEMATOLOGICA, 2017, 102 : 82 - 83
[14] RETRACTED: Kidney Injury Molecule-1 Is Up-Regulated in Renal Epithelial Cells in Response to Oxalate In Vitro and in Renal Tissues in Response to Hyperoxaluria In Vivo (Retracted Article)
Khandrika, Lakshmipathi
Koul, Sweaty
Meacham, Randall B.
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PLOS ONE, 2012, 7 (09):
[15] S-FIMBRIAE OF UROPATHOGENIC ESCHERICHIA-COLI BIND TO PRIMARY HUMAN RENAL PROXIMAL TUBULAR EPITHELIAL-CELLS BUT DO NOT INDUCE EXPRESSION OF INTERCELLULAR-ADHESION MOLECULE-1
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[16] Kidney Injury Molecule-1 Is Up-Regulated in Renal Epithelial Cells in Response to Oxalate In Vitro and in Renal Tissues in Response to Hyperoxaluria In Vivo (vol 15, e0234862, 2020)
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[17] RETRACTION: Kidney Injury Molecule-1 Is Up-Regulated in Renal Epithelial Cells in Response to Oxalate In Vitro and in Renal Tissues in Response to Hyperoxaluria In Vivo (Retraction of Vol 7, art no E44174, 2012)
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PLOS ONE, 2020, 15 (06):
[18] lncRNA NORAD alleviates dysfunction of renal proximal tubular epithelial cells during the sepsis-associated acute kidney injury by modulating the miR-155-5p-PDK1 axis
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[20] Suppression of chemically induced apoptosis but not necrosis of renal proximal tubular epithelial (LLC-PK1) cells by focal adhesion kinase (FAK) - Role of FAK in maintaining focal adhesion organization after acute renal cell injury
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