Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell- derived cardiomyocytes

被引：14
|
作者：
Zhang, Joe Z. [1 ,2 ]
Zhao, Shane Rui [1 ,2 ]
Tu, Chengyi [1 ,2 ]
Pang, Paul [1 ,2 ]
Zhang, Mao [1 ,2 ]
Wu, Joseph C. [1 ,2 ,3 ]
机构：
[1] Stanford Univ, Stanford Cardiovasc Inst, Sch Med, Stanford, CA 94305 USA
[2] Stanford Univ, Sch Med, Dept Med, Div Cardiovasc Med, Stanford, CA 94305 USA
[3] Stanford Univ, Sch Med, Dept Radiol, Stanford, CA 94305 USA
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 04期
关键词：
Cell Biology; Cell culture; Microscopy; Stem Cells;
D O I：
10.1016/j.xpro.2021.100859
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca2+) handling, and action potential.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).
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页数：16
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