Ratiometric Detection of Mitochondrial Thiols with a Two-Photon Fluorescent Probe

被引：342
|
作者：
Lim, Chang Su [3 ]
Masanta, Goutam [1 ]
Kim, Hyung Joong [2 ]
Han, Ji Hee [3 ]
Kim, Hwan Myung [1 ,2 ]
Cho, Bong Rae [3 ]
机构：
[1] Ajou Univ, Mol Sci & Technol Res Ctr, Suwon 443749, South Korea
[2] Ajou Univ, Div Energy Syst Res, Suwon 443749, South Korea
[3] Korea Univ, Dept Chem, Seoul 136701, South Korea
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2011年 / 133卷 / 29期
关键词：
GLUTATHIONE; ANTIOXIDANTS; HOMOCYSTEINE; BOND; CELL;
D O I：
10.1021/ja205081s
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report a ratiometric two-photon probe (SSH-Mito) for mitochondrial thiols. This probe shows a marked blue-to-yellow emission color change in response to RSH, a significant two-photon cross section, good mitochondrial thiol selectivity, low cytotoxicity, and insensitivity to pH over the biologically relevant pH range, allowing the direct visualization of RSH levels in live cells as well as in living tissues at 90-190 mu m depth without interference from other biologically relevant species through the use of two-photon microscopy.
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页码：11132 / 11135
页数：4
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