Perspectives on microbial cell surface display in bioremediation

被引:53
|
作者
Salem, M. [1 ,3 ]
Brim, H. [2 ]
Hussain, S. [3 ]
Arshad, M. [3 ]
Leigh, M. B. [4 ]
Zia-ul-hassan [3 ]
机构
[1] UFZ Helmholtz Ctr Environm Res, Helmholtz Ctr Environm Res, Dept Environm Microbiol, D-04318 Leipzig, Germany
[2] Howard Univ, Dept Microbiol & Canc Ctr, Washington, DC 20060 USA
[3] Univ Agr Faisalabad, Inst Soil & Environm Sci, Faisalabad 38040, Pakistan
[4] Univ Alaska, Inst Arctic Biol, Fairbanks, AK 99775 USA
关键词
heterologous proteins; cell surface display; bacteria; yeast; bioremediation;
D O I
10.1016/j.biotechadv.2007.10.002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The display of heterologous proteins on the microbial cell surface by means of recombinant DNA biotechnologies has emerged as a novel approach for bioremediation of contaminated sites. Both bacteria and yeasts have been investigated for this purpose. Cell surface expression of specific proteins allows the engineered microorganisms to transport, bio-accumulate and/or detoxify heavy metals as well as to degrade xenobiotics. These otherwise would not be taken up and transformed by the microbial cell. This review focuses on the application of cell surface displays for the enhanced bio-accumulation of heavy metals by metal binding proteins. It also reviews the biodegradation of xenobiotics by enzymes/proteins expressed on microbial cell surfaces. (C) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:151 / 161
页数:11
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