In vitro evaluation of the acquisition of resistance, antifungal activity and synergism of Brazilian red propolis with antifungal drugs on Candida spp.

被引:53
|
作者
Pippi, B. [1 ]
Lana, A. J. D. [2 ]
Moraes, R. C. [2 ]
Gueez, C. M. [3 ]
Machado, M. [3 ]
de Oliveira, L. F. S. [3 ]
Lino von Poser, G. [2 ]
Fuentefria, A. M. [1 ,2 ]
机构
[1] Univ Fed Rio Grande do Sul, Programa Pos Grad Microbiol Agr & Ambiente, Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande do Sul, Programa Pos Grad Ciencias Farmaceut, Porto Alegre, RS, Brazil
[3] Univ Fed Pampa, Programa Pos Grad Ciencias Farmaceut, Uruguaiana, Brazil
关键词
antifungal agent; Brazilian red propolis; Candida; fluconazole; resistance; synergism; HUMAN-IMMUNODEFICIENCY-VIRUS; MESSENGER-RNA LEVELS; FLUCONAZOLE RESISTANCE; AZOLE RESISTANCE; STAPHYLOCOCCUS-AUREUS; SALMONELLA-TYPHI; ALBICANS; EPIDEMIOLOGY; MECHANISMS; ANTIBACTERIAL;
D O I
10.1111/jam.12746
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
AimsTo evaluate the ability of Candida parapsilosis and Candida glabrata to develop phenotypic resistance to a benzophenone enriched fraction obtained from Brazilian red propolis (BZP-BRP) as compared to fluconazole (FLC). To investigate possible synergy between BZP-BRP and FLC and anidulafungin (AND). Methods and ResultsTo analyse the development of resistance, isolates susceptible to these antifungals were cultured in increasing concentrations of FLC and BZP-BRP. The increase in FLC minimum inhibitory concentration for all isolates was evident and the majority developed resistance, whereas none isolated became less susceptible to BZP-BRP. Synergism was investigated by checkerboard method. BZP-BRP demonstrated synergy with FLC and indifference with AND for most isolates. ConclusionsIn conclusion, the synergism observed with FLC suggests that BZP-BRP could be a possible therapeutic strategy for the treatment of infections related to FLC-resistant Candida sp. Significance and Impact of the StudyThe indiscriminate use of antifungals results in the emergence of drug-resistant strains among previously susceptible populations. BZP-BRP can become an alternative for the treatment of persistent infections caused by Candida sp.
引用
收藏
页码:839 / 850
页数:12
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