Magnetic beads-based electrochemiluminescence assay for rapid and sensitive detection of telornerase activity

被引：32
|
作者：
Zhou, Xiaoming
Xing, Da [1 ]
Zhu, Debin
Jia, Li
机构：
[1] S China Normal Univ, MOE Key Lab Laser Life Sci, Guangzhou 510631, Peoples R China
来源：
ELECTROCHEMISTRY COMMUNICATIONS | 2008年 / 10卷 / 04期
基金：
中国国家自然科学基金;
关键词：
telomerase activity; TRAP; electrochemiluminescence; magnetic beads;
D O I：
10.1016/j.elecom.2008.01.039
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
Telomerase is a potential cancer marker. We developed a new and robust telomerase activity assay which combines the modified telomere repeat amplification protocol (TRAP) with magnetic beads-based electrochemiluminescence (ECL) detection. The high performance of this assay is related to the determination of telomerase activity from single cell levels, and ECL intensity is linear over the range of 1-1000 HeLa cell equivalents. The proposed telomerase assay offers a highly cost- and time-effective alternative to presently available telomerase assays, which are limited by tedious and complicated post-PCR detection. (c) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：564 / 567
页数：4
相关论文
共 50 条
  • [21] Establishment of magnetic beads-based enzyme immunoassay for detection of chloramphenicol in milk
    Xu, Jing
    Yin, Weiwei
    Zhang, Yuanyang
    Yi, Jian
    Meng, Meng
    Wang, Yabin
    Xue, Huyin
    Zhang, Taichang
    Xi, Rimo
    FOOD CHEMISTRY, 2012, 134 (04) : 2526 - 2531
  • [22] Rapid Quantitative Detection of Salmonella spp. via Magnetic Beads-based Fluorescent Lateral Flow Immunoassay
    Zhuang, Linlin
    Gong, Jiansen
    Ji, Yongxin
    Gu, Ning
    Zhang, Yu
    2019 IEEE 19TH INTERNATIONAL CONFERENCE ON NANOTECHNOLOGY (IEEE-NANO 2019), 2019, : 80 - 85
  • [23] Development of a novel multiplex beads-based assay for autoantibody detection for colorectal cancer diagnosis
    Villar-Vazquez, Roi
    Padilla, Guillermo
    Jesus Fernandez-Acenero, Maria
    Suarez, Adolfo
    Fuente, Eduardo
    Pastor, Carlos
    Calero, Miguel
    Barderas, Rodrigo
    Ignacio Casal, J.
    PROTEOMICS, 2016, 16 (08) : 1280 - 1290
  • [24] Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots
    Krejcova, Ludmila
    Nejdl, Lukas
    Hynek, David
    Krizkova, Sona
    Kopel, Pavel
    Adam, Vojtech
    Kizek, Rene
    MOLECULES, 2013, 18 (12): : 15573 - 15586
  • [25] Quantification of rHuEPO-α by magnetic beads-based aptameric real-time PCR assay
    Tang, Jijun
    Guo, Lei
    Shen, Rui
    Yu, Tao
    Xu, Hua
    Liu, Hezhu
    Ma, Xiaoxi
    Xie, Jianwei
    ANALYST, 2010, 135 (11) : 2924 - 2929
  • [26] MAGNETIC BEADS-BASED MICROFLUIDIC SYSTEMS: THE PAST AND THE FUTURE
    Bilkova, Zuzana
    Svobodova, Zuzana
    CECE 2012: 9TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS, 2012, : 97 - 98
  • [27] Magnetic Bead and Nanoparticle Based Electrochemiluminescence Amplification Assay for Direct and Sensitive Measuring of Telomerase Activity
    Zhou, Xiaoming
    Xing, Da
    Zhu, Debin
    Jia, Li
    ANALYTICAL CHEMISTRY, 2009, 81 (01) : 255 - 261
  • [28] Magnetic beads-based electrochemical immunosensor for detection of pseudorabies virus antibody in swine serum
    Li, Fang
    Zhou, Rui
    Zhao, Kaihong
    Chen, Huanchun
    Hu, Yonggang
    TALANTA, 2011, 87 : 302 - 306
  • [29] Magnetic beads-based electrochemiluminescence immunosensor for determination of cancer markers using quantum dot functionalized PtRu alloys as labels
    Zhang, Yan
    Ge, Shenguang
    Wang, Shaowei
    Yan, Mei
    Yu, Jinghua
    Song, Xianrang
    Liu, Weiyan
    ANALYST, 2012, 137 (09) : 2176 - 2182
  • [30] Magnetic Beads-Based Electrochemical Sensors Applied to the Detection and Quantification of Bioterrorism/Biohazard Agents
    Pedrero, Maria
    Campuzano, Susana
    Pingarron, Jose M.
    ELECTROANALYSIS, 2012, 24 (03) : 470 - 482
12345