Yeast two-hybrid identification of prostatic proteins interacting with human sex hormone-binding globulin

被引：14
|
作者：
Pope, SN [1 ]
Lee, IR [1 ]
机构：
[1] Curtin Univ Technol, Sch Biomed Sci, Perth, WA 6845, Australia
来源：
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2005年 / 94卷 / 1-3期
关键词：
yeast two-hybrid; sex hormone-binding globulin; prostate;
D O I：
10.1016/j.jsbmb.2005.01.007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Yeast two-hybrid (Y2H) screening of a prostate cDNA library with the cDNA for sex hormone-binding globulin (SHBG) has been used to identify proteins through which SHBG may exert autocrine or paracrine effects on sex steroid target tissues. The library screen gave 230 positive interactions of which around 60 have been sequenced. Of the proteins identified to date from database (BLAST) searches of these sequences, SHBG is one of those occurring most frequently. Amongst the proteins of interest are the membrane-associated proteins flotillin-1 and PRV-1, the enzymes cathepsin D, kallikrein 4 and acid phosphatase, various metallothioneins and translation elongation factor 1 alpha. The significance of the interaction of SHBG with these proteins is discussed. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码：203 / 208
页数：6
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