Comparison of Two Mycoplasma genitalium Real-Time PCR Detection Methodologies

被引：31
|
作者：
Twin, Jimmy [1 ,2 ]
Taylor, Nicole [1 ,2 ]
Garland, Suzanne M. [1 ,2 ,3 ,4 ]
Hocking, Jane S. [5 ]
Walker, Jennifer [5 ]
Bradshaw, Catriona S. [6 ,7 ]
Fairley, Christopher K. [5 ,6 ]
Tabrizi, Sepehr N. [1 ,2 ,3 ]
机构：
[1] Royal Womens Hosp, Dept Microbiol & Infect Dis, Melbourne, Vic, Australia
[2] Murdoch Childrens Res Inst, Melbourne, Vic, Australia
[3] Univ Melbourne, Dept Obstet & Gynaecol, Melbourne, Vic 3010, Australia
[4] Royal Childrens Hosp, Dept Microbiol, Melbourne, Vic, Australia
[5] Univ Melbourne, Melbourne Sch Populat Hlth, Melbourne, Vic 3010, Australia
[6] Melbourne Sexual Hlth Ctr, Melbourne, Vic, Australia
[7] Monash Univ, Dept Epidemiol & Prevent Med, Melbourne, Vic 3004, Australia
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 03期
关键词：
QUANTITATIVE DETECTION; CHLAMYDIA-TRACHOMATIS; URETHRITIS; ASSAY; SPECIMENS; URINE; GENE; MEN; AMPLIFICATION; SENSITIVITY;
D O I：
10.1128/JCM.02328-10
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399).
引用
收藏
页码：1140 / 1142
页数：3
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