Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

被引：3
|
作者：
Mun, Dasom [1 ]
Kang, Ji-Young [1 ]
Chun, Yumin [1 ]
Park, Da-Seul [1 ]
Kim, Hyoeun [2 ]
Yun, Nuri [3 ]
Lee, Seung-Hyun [2 ]
Joung, Boyoung [1 ]
机构：
[1] Yonsei Univ, Coll Med, Div Cardiol, 50-1 Yonsei Ro, Seoul 03722, South Korea
[2] Yonsei Univ, Coll Med, Dept Biochem & Mol Biol, 50-1 Yonsei Ro, Seoul 03722, South Korea
[3] Yonsei Univ, Inst Life Sci & Biotechnol, 50 Yonsei Ro, Seoul 03722, South Korea
来源：
STEM CELL RESEARCH | 2022年 / 65卷
基金：
新加坡国家研究基金会;
关键词：
D O I：
10.1016/j.scr.2022.102940
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
PITX2 is a homeobox gene located in the human 4q25 locus and is commonly associated with atrial fibrillation (AF). Here, we generated two PITX2 knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 genome editing. The edited iPSCs maintained full pluripotency, normal karyotype and sponta-neous differentiation capability. This cell line provides a suitable model for investigating the physiopathology of PITX2 mutation in atrial fibrillation.
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页数：4
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