A quantum-dot based protein module for in vivo monitoring of protease activity through fluorescence resonance energy transfer

被引：36

作者：

Biswas, Payal ^{[2
]}

Cella, Lakshmi N. ^{[1
,3
]}

Kang, Seung Hyun ^{[2
]}

Mulchandani, Ashok ^{[2
]}

Yates, Marylynn V. ^{[4
]}

Chen, Wilfred ^{[1
]}

机构：

[1] Univ Delaware, Dept Chem Engn, Newark, DE 19716 USA

[2] Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA

[3] Univ Calif Riverside, Cell Mol & Dev Biol Grad Program, Riverside, CA 92521 USA

[4] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 18期

基金：

美国国家科学基金会;

关键词：

IMMUNODEFICIENCY-VIRUS TYPE-1; MATRIX METALLOPROTEINASES; ELASTIN-PROTEIN; HIV-1; PROTEASE; INHIBITORS; RESISTANCE; TARGETS; PHASE; VPR;

D O I：

10.1039/c1cc10648a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Here, we present a new generation of nanoscale probes for in vivo monitoring of protease activity by fluorescence resonance energy transfer (FRET). The approach is based on a genetically programmable protein module carrying a fluorescently labeled, protease-specific sequence that can self-assemble onto quantum dots. The protein module was used for real-time detection of human immunodeficiency virus type-1 protease (HIV-1 Pr) activity as well as quantitative assessment of inhibitor efficiency.

引用

页码：5259 / 5261

页数：3

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