Multi-photon excitation of fluorescence

被引：2
|
作者：
Lakowicz, JR [1 ]
Gryczynski, I [1 ]
机构：
[1] Univ Maryland, Sch Med, Ctr Fluorescence Spect, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA
来源：
ADVANCES IN OPTICAL BIOPHYSICS, PROCEEDINGS OF | 1998年 / 3256卷
关键词：
D O I：
10.1117/12.307075
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
The increasing availability of fs lasers has resulted in expanded interest in multi-photon excitation of fluorescence. In this overview paper, we describe recent developments in multi-photon induced fluorescence spectroscopy and anisotropy. Experimental results are presented for three-photon excitation of tyrosine and tryptohan, and for the single tryptophan protein, troponin C Mutant F22. Three-photon excitation has been also applied to study DPH-labeled membranes and DNA stained with DAPI. The enhancement in spatial resolution resulting from three-photon excitation in spectroscopic and microscopic conditions is described for BBO scintillator in solution. We also describe two-photon excitation with using two-photon at different wavelengths.
引用
收藏
页码：24 / 37
页数：14
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