Entertoxin and tonic shock syndrome toxin-1 production by Staphylococcus aureus isolated from bovine mastitis

One hundred and four Staphylococcus aureus strains isolated from cows affected with mastitis from 19 herds in six different provinces of Poland were examined for the production of staphylococcal enterotoxins (SEs) A, SEB, SEC and SED and toxic shock syndrome toxin-1 (TSST-1) production by reversed-passive latex agglutination (RPLA) method. The presence of genes coding the production of these toxins was determined by PCR. Twenty three of the isolates produced one or two toxins. Nine strains produced SEA, 1 produced SEB, four produced SECbovine, seven produced SED and 2 produced TSST-I and SEC. The results indicated a complete correlation between the amplification and RPLA. Two multiplex polymerase chain reactions were developed to shorten the time of detection of toxigenic strains of S. aureus.