A vernalization protocol for obtaining progenies from regenerated and transgenic tall fescue plants

被引：8
|
作者：
Wang, ZY [1 ]
Bell, J [1 ]
Scott, M [1 ]
机构：
[1] Samuel Roberts Noble Fdn Inc, Forage Biotechnol Grp, Ardmore, OK 73401 USA
来源：
PLANT BREEDING | 2003年 / 122卷 / 06期
关键词：
Festuca arundinacea; forage and turf grass; plant regeneration; transgenic plants; vernalization;
D O I：
10.1111/j.1439-0523.2003.00879.x
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Tall fescue is an important outcrossing forage and turf grass species that requires vernalization to flower. A reproducible protocol was developed for vernalization of regenerated, transgenic and seed-derived tall fescue plants. Following the vernalization scheme that involved gradual changes of temperature and daylength, seeds were routinely produced from vernalized plants under greenhouse conditions. Molecular analyses of progenies obtained from crosses between transgenic and seed-derived plants revealed stable meiotic transmission of transgenes following Mendelian inheritance in transgenic tall fescue.
引用
收藏
页码：536 / 538
页数：3
相关论文
共 50 条
  • [21] THE EFFECT OF SELECTION PRESSURE ON TRANSFORMATION FREQUENCY AND COPY NUMBER IN TRANSGENIC PLANTS OF TALL FESCUE (FESTUCA-ARUNDINACEA SCHREB)
    DALTON, SJ
    BETTANY, AJE
    TIMMS, E
    MORRIS, P
    PLANT SCIENCE, 1995, 108 (01) : 63 - 70
  • [22] Expression of a sulphur-rich sunflower albumin gene in transgenic tall fescue (Festuca arundinacea Schreb.) plants
    Z.Y. Wang
    X.D. Ye
    J. Nagel
    I. Potrykus
    G. Spangenberg
    Plant Cell Reports, 2001, 20 : 213 - 219
  • [23] Expression of a sulphur-rich sunflower albumin gene in transgenic tall fescue (Festuca arundinacea Schreb.) plants
    Wang, ZY
    Ye, XD
    Nagel, J
    Potrykus, I
    Spangenberg, G
    PLANT CELL REPORTS, 2001, 20 (03) : 213 - 219
  • [24] Enhanced tolerance of transgenic tall fescue plants overexpressing 2-Cys peroxiredoxin against methyl viologen and heat stresses
    Kyung-Hee Kim
    Iftekhar Alam
    Ki-Won Lee
    Shamima Akhtar Sharmin
    Sang-Soo Kwak
    Sang Yeol Lee
    Byung-Hyun Lee
    Biotechnology Letters, 2010, 32 : 571 - 576
  • [25] Enhanced tolerance of transgenic tall fescue plants overexpressing 2-Cys peroxiredoxin against methyl viologen and heat stresses
    Kim, Kyung-Hee
    Alam, Iftekhar
    Lee, Ki-Won
    Sharmin, Shamima Akhtar
    Kwak, Sang-Soo
    Lee, Sang Yeol
    Lee, Byung-Hyun
    BIOTECHNOLOGY LETTERS, 2010, 32 (04) : 571 - 576
  • [26] Transgenic Japanese lawngrass (Zoysia japonica Steud) plants regenerated from protoplasts
    Inokuma, C
    Sugiura, K
    Imaizumi, N
    Cho, C
    PLANT CELL REPORTS, 1998, 17 (05) : 334 - 338
  • [27] Erratum to: Enhanced tolerance of transgenic tall fescue plants overexpressing 2-Cys peroxiredoxin against methyl viologen and heat stresses
    Kyung-Hee Kim
    Iftekhar Alam
    Ki-Won Lee
    Shamima Akhtar Sharmin
    Sang-Soo Kwak
    Sang Yeol Lee
    Byung-Hyun Lee
    Biotechnology Letters, 2010, 32 (4) : 577 - 577
  • [28] Comparative analysis of transgenic tall fescue (Festuca arundinacea Schreb.) plants obtained by Agrobacterium-mediated transformation and particle bombardment
    Gao, Caixia
    Long, Danfeng
    Lenk, Ingo
    Nielsen, Klaus Kristian
    PLANT CELL REPORTS, 2008, 27 (10) : 1601 - 1609
  • [29] Comparative analysis of transgenic tall fescue (Festuca arundinacea Schreb.) plants obtained by Agrobacterium-mediated transformation and particle bombardment
    Caixia Gao
    Danfeng Long
    Ingo Lenk
    Klaus Kristian Nielsen
    Plant Cell Reports, 2008, 27 : 1601 - 1609
  • [30] Transgenic Japanese lawngrass (Zoysia japonica Steud.) plants regenerated from protoplasts
    C. Inokuma
    K. Sugiura
    N. Imaizumi
    C. Cho
    Plant Cell Reports, 1998, 17 : 334 - 338
12345