Use of PCR for detecting Aspergillus flavus in maize treated by gamma radiation process

被引：2
|
作者：
Aquino, Simone [1 ]
Greiner, Ralf [3 ]
Konietzny, Ursula [3 ]
Hassegawa, Regina H. [2 ]
dos Reis, Tatiana Alves [2 ]
Correa, Benedito [2 ]
Villavicencio, Anna Lucia C. H. [1 ]
机构：
[1] IPEN CNEN SP, Sao Paulo, Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, Brazil
[3] Ctr Mol Biol, Fed Res Ctr Nutr & Food, Karlsruhe, Germany
来源：
FOOD BIOTECHNOLOGY | 2008年 / 22卷 / 1-2期
基金：
巴西圣保罗研究基金会;
关键词：
gamma radiation; aspergillus flavus; aflatoxin; maize; PCR; DNA;
D O I：
10.1080/08905430802043164
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The aim of this study was to verify the effects of gamma radiation process on the fungal DNA and the application of PCR in the detection of Aspergillus flavus in irradiated maize grains. The samples were inoculated with a toxigenic strain and incubated under controlled conditions of relative humidity, water activity, and temperature for 15 days. After incubation, the samples were treated with gamma radiation with doses of 5 and 10 kGy and individually analyzed. The use of PCR technique showed the presence of DNA bands of Aspergillus flavus in all irradiated samples that showed no fungal growth in agar medium.
引用
收藏
页码：150 / 159
页数：10
相关论文
共 35 条
  • [21] Use of microsatellite markers to assess the competitive ability of nontoxigenic Aspergillus flavus strains in studies on biocontrol of aflatoxins in maize in Thailand
    Tran-Dinh, N.
    Pitt, J. I.
    Markwell, P. J.
    BIOCONTROL SCIENCE AND TECHNOLOGY, 2018, 28 (03) : 215 - 225
  • [22] INFLUENCE OF INOCULUM SIZE OF ASPERGILLUS-FLAVUS LINK ON THE PRODUCTION OF AFLATOXIN-B1 IN MAIZE MEDIUM BEFORE AND AFTER EXPOSURE TO COMBINATION TREATMENT OF HEAT AND GAMMA-RADIATION
    ODAMTTEN, GT
    APPIAH, V
    LANGERAK, DI
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1987, 4 (02) : 119 - 127
  • [23] GAMMA-RADIATION IN THE PREPARATION OF MAIZE SILAGE FOR STUDIES ON THE AEROBIC DETERIORATION PROCESS
    WOOLFORD, MK
    JOURNAL OF AGRICULTURAL SCIENCE, 1983, 101 (DEC): : 711 - 716
  • [24] STUDIES ON ASPERGILLUS-FLAVUS .2. RESPONSES OF GERMINATING CONIDIA TO SINGLE AND COMBINED TREATMENTS OF GAMMA-RADIATION AND HEAT
    PADWALDESAI, SR
    GHANEKAR, AS
    SREENIVASAN, A
    ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 1976, 16 (01) : 53 - 57
  • [25] The use of linear attenuation coefficients of gamma radiation for detecting knots in Pinus radiata
    Karsulovic, JT
    Leon, LA
    Dinator, MI
    FOREST PRODUCTS JOURNAL, 1999, 49 (02) : 73 - 76
  • [26] Use of Mycological, nested PCR, and Real-time PCR Methods on BAL Fluids for Detection of Aspergillus fumigatus and A. flavus in Solid Organ Transplant Recipients
    Hossein Zarrinfar
    Hossein Mirhendi
    Koichi Makimura
    Kazuo Satoh
    Hossein Khodadadi
    Omolbanin Paknejad
    Mycopathologia, 2013, 176 : 377 - 385
  • [27] Use of Mycological, nested PCR, and Real-time PCR Methods on BAL Fluids for Detection of Aspergillus fumigatus and A-flavus in Solid Organ Transplant Recipients
    Zarrinfar, Hossein
    Mirhendi, Hossein
    Makimura, Koichi
    Satoh, Kazuo
    Khodadadi, Hossein
    Paknejad, Omolbanin
    MYCOPATHOLOGIA, 2013, 176 (5-6) : 377 - 385
  • [28] USE OF DIETS TREATED WITH GAMMA-RADIATION IN FEEDING RAINBOW-TROUT
    GRUMBERG, J
    GONZALEZ, O
    ARCHIVOS LATINOAMERICANOS DE NUTRICION, 1984, 34 (01) : 83 - 93
  • [29] The Development of a qPCR Assay to Measure Aspergillus flavus Biomass in Maize and the Use of a Biocontrol Strategy to Limit Aflatoxin Production (vol 11, 179, 2019)
    Mitema, Alfred
    Okoth, Sheila
    Rafudeen, Suhail M.
    TOXINS, 2019, 11 (07)
  • [30] Use of gamma radiation in sheep butter manufacturing process for shelf-life extension
    Santos, Ines Carvalho
    Pinto, Joana
    Pimenta, Andreia I.
    Madureira, Joana
    Matos, Paula
    Viegas, Carla
    Raposo, Antonio
    Margaca, Fernanda M. A.
    Verde, Sandra Cabo
    INTERNATIONAL DAIRY JOURNAL, 2017, 71 : 43 - 49
1234