Use of PCR for detecting Aspergillus flavus in maize treated by gamma radiation process

被引：2
|
作者：
Aquino, Simone [1 ]
Greiner, Ralf [3 ]
Konietzny, Ursula [3 ]
Hassegawa, Regina H. [2 ]
dos Reis, Tatiana Alves [2 ]
Correa, Benedito [2 ]
Villavicencio, Anna Lucia C. H. [1 ]
机构：
[1] IPEN CNEN SP, Sao Paulo, Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, Brazil
[3] Ctr Mol Biol, Fed Res Ctr Nutr & Food, Karlsruhe, Germany
来源：
FOOD BIOTECHNOLOGY | 2008年 / 22卷 / 1-2期
基金：
巴西圣保罗研究基金会;
关键词：
gamma radiation; aspergillus flavus; aflatoxin; maize; PCR; DNA;
D O I：
10.1080/08905430802043164
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The aim of this study was to verify the effects of gamma radiation process on the fungal DNA and the application of PCR in the detection of Aspergillus flavus in irradiated maize grains. The samples were inoculated with a toxigenic strain and incubated under controlled conditions of relative humidity, water activity, and temperature for 15 days. After incubation, the samples were treated with gamma radiation with doses of 5 and 10 kGy and individually analyzed. The use of PCR technique showed the presence of DNA bands of Aspergillus flavus in all irradiated samples that showed no fungal growth in agar medium.
引用
收藏
页码：150 / 159
页数：10
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