Molecular and biochemical properties of two P1B2-ATPases, CsHMA3 and CsHMA4, from cucumber

被引:24
|
作者
Migocka, Magdalena [1 ]
Papierniak, Anna [1 ]
Maciaszczyk-Dziubinska, Ewa [2 ]
Posyniak, Ewelina [1 ]
Kosieradzka, Anna [1 ]
机构
[1] Univ Wroclaw, Dept Plant Mol Physiol, Inst Expt Biol, PL-50328 Wroclaw, Poland
[2] Univ Wroclaw, Dept Genet & Cell Physiol, Inst Expt Biol, PL-50328 Wroclaw, Poland
来源
PLANT CELL AND ENVIRONMENT | 2015年 / 38卷 / 06期
关键词
detoxification; genome; heavy metals; heavy metal ATPases; plasma membrane; tonoplast; P-TYPE ATPASE; HEAVY-METAL ATPASE; SACCHAROMYCES-CEREVISIAE; PLASMA-MEMBRANE; P-1B-TYPE ATPASE; ZINC HOMEOSTASIS; YEAST COPPER; CD TOLERANCE; ARABIDOPSIS; EXPRESSION;
D O I
10.1111/pce.12447
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
P-1B-ATPases (heavy metal ATPases, HMAs) constitute a multigenic subfamily of P-ATPases involved in the transport of monovalent and divalent heavy metals in plant cells. Here, we present the organization of genes encoding the HMA family in the cucumber genome and report the function and biochemical properties of two cucumber proteins homologous to the HMA2-4-like plant HMAs. Eight genes encoding putative P-1B-ATPases were identified in the cucumber genome. Among them, CsHMA3 was predominantly expressed in roots and up-regulated by Pb, Zn and Cd excess, whereas the CsHMA4 transcript was most abundant in roots and flowers of cucumber plants, and elevated under Pb and Zn excess. Expression of CsHMA3 in Saccharomyces cerevisiae enhanced yeast tolerance to Cd and Pb, whereas CsHMA4 conferred increased resistance of yeast cells to Cd and Zn. Immunostaining with specific antibodies raised against cucumber proteins revealed tonoplast localization of CsHMA3 and plasma membrane localization of CsHMA4 in cucumber root cells. Kinetic studies of CsHMA3 and CsHMA4 in yeast membranes indicated differing heavy metal cation affinities of these two proteins. Altogether, the results suggest an important role of CsHMA3 and CsHMA4 in Cd and Pb detoxification and Zn homeostasis in cucumber cells.
引用
收藏
页码:1127 / 1141
页数:15
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