An improved method for the separation of lichen symbionts

被引：0
|
作者：
Fontaniella, B [1 ]
Molina, MC [1 ]
Vicente, C [1 ]
机构：
[1] Univ Complutense, Fac Biol, Lichen Team, Lab Plant Physiol, E-28040 Madrid, Spain
来源：
PHYTON-ANNALES REI BOTANICAE | 2000年 / 40卷 / 02期
关键词：
Evernia prunastri; Lichenes; photobionts; symbionts; isolation technique;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
A novel method for the isolation of lichen photobionts by density gradient centrifugation has been assessed using the lichen Evernia prunastri as an experimental model. An initial sucrose-KI gradient was prepared in which algae and small hyphal fragments formed an interphase in a sucrose-KI gradient. Then, 10 mM phosphate buffer is added and the preparation centrifuged a second time. This partitioned the algal cells towards the buffer while the bulk of the fungal hyphae were retained in the sucrose solution. This method allowed the purification of algal cells with no contamination from the fungal partner.
引用
收藏
页码：323 / 328
页数：6
相关论文
共 50 条
  • [41] An Improved GraDe Method for Blind Separation of Graph Signals
    Sadeghi, Mohammad
    Babaie-Zadeh, Massoud
    IEEE TRANSACTIONS ON SIGNAL PROCESSING, 2023, 71 : 4382 - 4391
  • [42] THE RHEOLOGY OF LEUKOCYTE SUBPOPULATIONS - A NEW IMPROVED SEPARATION METHOD
    CIUFFETTI, G
    RIZZO, MT
    MERCURI, M
    PALTRICCIA, R
    LOMBARDINI, R
    HAEMATOLOGICA, 1989, 74 (02) : 223 - 224
  • [43] A microsampling method for genotyping coral symbionts
    D. W. Kemp
    W. K. Fitt
    G. W. Schmidt
    Coral Reefs, 2008, 27 : 289 - 293
  • [44] A microsampling method for genotyping coral symbionts
    Kemp, D. W.
    Fitt, W. K.
    Schmidt, G. W.
    CORAL REEFS, 2008, 27 (02) : 289 - 293
  • [45] Photosynthetic symbionts in Antarctic terrestrial ecosystems: the physiological response of lichen photobionts to drought and cold
    Sadowsky, Andres
    Ott, Sieglinde
    SYMBIOSIS, 2012, 58 (1-3) : 81 - 90
  • [46] Life at the Limits: Capacities of Isolated and Cultured Lichen Symbionts to Resist Extreme Environmental Stresses
    J.-P. de Vera
    P. Rettberg
    S. Ott
    Origins of Life and Evolution of Biospheres, 2008, 38 : 457 - 468
  • [47] Photosynthetic symbionts in Antarctic terrestrial ecosystems: the physiological response of lichen photobionts to drought and cold
    Andres Sadowsky
    Sieglinde Ott
    Symbiosis, 2012, 58 : 81 - 90
  • [48] Co-dispersal of symbionts in the lichen Cladonia stellaris inferred from genomic data
    Alonso-Garcia, Marta
    Pino-Bodas, Raquel
    Villarreal, A. Juan Carlos
    FUNGAL ECOLOGY, 2022, 60
  • [49] Separation of vibration signal content using an improved discrete-random separation method
    Peeters, C.
    Antoni, J.
    Daems, P-J
    Helsen, J.
    PROCEEDINGS OF INTERNATIONAL CONFERENCE ON NOISE AND VIBRATION ENGINEERING (ISMA2020) / INTERNATIONAL CONFERENCE ON UNCERTAINTY IN STRUCTURAL DYNAMICS (USD2020), 2020, : 745 - 753
  • [50] Symbionts out of sync: Decoupled physiological responses are widespread and ecologically important in lichen associations
    Meyer, Abigail R.
    Koch, Natalia M.
    McDonald, Tami
    Stanton, Daniel E.
    SCIENCE ADVANCES, 2024, 10 (24):
12345