A colorimetric paper-based sensor for toltrazuril and its metabolites in feed, chicken, and egg samples

被引：56
|
作者：
Wang, Zhongxing [1 ,2 ,3 ]
Zhang, Jian [4 ]
Liu, Liqiang [1 ,2 ,3 ]
Wu, Xiaoling [1 ,2 ,3 ]
Kuang, Hua [1 ,2 ,3 ]
Xu, Chuanlai [1 ,2 ,3 ]
Xu, Liguang [1 ,2 ,3 ]
机构：
[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi, Peoples R China
[2] Jiangnan Univ, Int Joint Res Lab Biointerface & Biodetect, Wuxi, Peoples R China
[3] Jiangnan Univ, Sch Food Sci & Technol, Wuxi, Peoples R China
[4] Second Peoples Hosp Wuxi, Dept Stomatol, 68 Zhong Shan Rd, Wuxi 214002, Peoples R China
来源：
FOOD CHEMISTRY | 2019年 / 276卷
关键词：
Monoclonal antibody; Immunochromatographic assay; Toltrazuril; Metabolite; Feed; Egg; Chicken; LATERAL-FLOW IMMUNOASSAY; IMMUNOCHROMATOGRAPHIC STRIP; MONOCLONAL-ANTIBODY; RAPID DETECTION; PHARMACOKINETICS; SULFONE; EFFICACY; DETECT; ASSAY;
D O I：
10.1016/j.foodchem.2018.10.047
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
In this study, a sensitive monoclonal antibody (mAb) against toltrazuril (Tol) was developed based on a novel hapten. The 50% inhibitory concentrations (IC50) of toltrazuril and its metabolites ranged from 2.19 ng/mL to 4.21 ng/mL. Based on this mAb, a colorimetric paper-based sensor was developed for the rapid screening of Tol and its metabolites in samples. The proposed assay has cutoff values of < 20 mu g/kg for Tol and 50 mu g/kg for Tol sulfone when evaluated with the naked eye, and the results could be obtained in 15 min. Quantitative results were obtained with a strip scan reader, with limits of detection < 2.60 mu g/kg for Tol and its metabolites in real samples. The sensitivity of both qualitative and quantitative detection meets the European Union requirements. Therefore, this strip assay provides a useful tool for the on-site detection and rapid initial screening of Tol and its derivatives in feed, egg, and chicken samples.
引用
收藏
页码：707 / 713
页数：7
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