A colorimetric paper-based sensor for toltrazuril and its metabolites in feed, chicken, and egg samples

被引：56

作者：

Wang, Zhongxing ^{[1
,2
,3
]}

Zhang, Jian ^{[4
]}

Liu, Liqiang ^{[1
,2
,3
]}

Wu, Xiaoling ^{[1
,2
,3
]}

Kuang, Hua ^{[1
,2
,3
]}

Xu, Chuanlai ^{[1
,2
,3
]}

Xu, Liguang ^{[1
,2
,3
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi, Peoples R China

[2] Jiangnan Univ, Int Joint Res Lab Biointerface & Biodetect, Wuxi, Peoples R China

[3] Jiangnan Univ, Sch Food Sci & Technol, Wuxi, Peoples R China

[4] Second Peoples Hosp Wuxi, Dept Stomatol, 68 Zhong Shan Rd, Wuxi 214002, Peoples R China

来源：

FOOD CHEMISTRY | 2019年 / 276卷

关键词：

Monoclonal antibody; Immunochromatographic assay; Toltrazuril; Metabolite; Feed; Egg; Chicken; LATERAL-FLOW IMMUNOASSAY; IMMUNOCHROMATOGRAPHIC STRIP; MONOCLONAL-ANTIBODY; RAPID DETECTION; PHARMACOKINETICS; SULFONE; EFFICACY; DETECT; ASSAY;

D O I：

10.1016/j.foodchem.2018.10.047

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study, a sensitive monoclonal antibody (mAb) against toltrazuril (Tol) was developed based on a novel hapten. The 50% inhibitory concentrations (IC50) of toltrazuril and its metabolites ranged from 2.19 ng/mL to 4.21 ng/mL. Based on this mAb, a colorimetric paper-based sensor was developed for the rapid screening of Tol and its metabolites in samples. The proposed assay has cutoff values of < 20 mu g/kg for Tol and 50 mu g/kg for Tol sulfone when evaluated with the naked eye, and the results could be obtained in 15 min. Quantitative results were obtained with a strip scan reader, with limits of detection < 2.60 mu g/kg for Tol and its metabolites in real samples. The sensitivity of both qualitative and quantitative detection meets the European Union requirements. Therefore, this strip assay provides a useful tool for the on-site detection and rapid initial screening of Tol and its derivatives in feed, egg, and chicken samples.

引用

页码：707 / 713

页数：7

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