Molecular cloning, expression, and functional characterization of novel mouse sulfotransferases

被引：43
|
作者：
Sakakibara, Y [1 ]
Yanagisawa, K
Takami, Y
Nakayama, T
Suiko, M
Liu, MC
机构：
[1] Univ Texas, Ctr Hlth, Dept Biochem, Tyler, TX 75710 USA
[2] Miyazaki Med Coll, Dept Biochem, Miyazaki 88916, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 247卷 / 03期
基金：
日本学术振兴会;
关键词：
D O I：
10.1006/bbrc.1998.8872
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Nucleotide sequences of two mouse cDNAs encoding new sulfotransferase enzymes were determined. Analysis of the deduced amino acid sequences revealed that one represents a novel member of the phenol sulfotransferase family and the other is highly homologous to human SULT2B1 hydroxysteroid sulfotransferases. The recombinant enzymes, transiently expressed in COS-7 cells, were characterized with respect to their substrate specificity using a variety of substrates for different types of sulfotransferases. The tissue-specific expression of these two new mouse sulfotransferases was examined by Northern blot analysis. (C) 1998 Academic Press.
引用
收藏
页码：681 / 686
页数：6
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