Isolation and characterization of 16 tetranucleotide microsatellite loci in the red panda (Ailurus fulgens)

被引:12
|
作者
Liang, Xu
Zhang, Zhi-He
Zhang, Liang
Zhang, Wen-Ping
Shen, Fu-Jun
Yang, Zhi
Hou, Rong
He, Wei
Wei, Kun
Yue, Bi-Song [1 ]
机构
[1] Sichuan Univ, Coll Life Sci, Minist Educ, Key Lab Bioresources & Ecoenvironm, Sichuan 610064, Peoples R China
[2] Chengdu Res Base Giant Panda Breeding, Key Lab Reprod & Conservat Genet Endangered Wildl, Chengdu 610081, Sichuan, Peoples R China
来源
MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 06期
关键词
Ailurus fulgens; (GATA) motif enrichment; microsatellite; red panda;
D O I
10.1111/j.1471-8286.2007.01759.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A total of 15 polymorphic tetranucleotide microsatellite loci in the red panda, Ailurus fulgens, were characterized in this study. Based on evaluations of 33 red pandas, the number of observed alleles for each locus ranged from seven to 17 and the expected and observed heterozygosities were 0.412-0.897 and 0.121-0.909, respectively. The mean polymorphic information content was 0.721. These markers would greatly strengthen the utilization of microsatellite tools in genetic variation studies in red panda populations. A total of 15 polymorphic tetranucleotide microsatellite loci in the red panda, Ailurus fulgens, were characterized in this study. Based on evaluations of 33 red pandas, the number of observed alleles for each locus ranged from seven to 17 and the expected and observed heterozygosities were 0.412-0.897 and 0.121-0.909, respectively. The mean polymorphic information content was 0.721. These markers would greatly strengthen the utilization of microsatellite tools in genetic variation studies in red panda populations.
引用
收藏
页码:1012 / 1014
页数:3
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