Paludirhabdus telluriireducens gen. nov., sp nov and Paludirhabdus pumila sp nov., isolated from soil of a mountain wetland and emended description of Gorillibacterium massiliense

Two strains of Gram-stain-positive, endospore-forming, motile by means of peritrichous flagella, aerobic or facultative anaerobic, and rod-shaped bacteria that were designated ON8(T) and ON6(T) were isolated from soil collected from a mountain wetland in Gwang-ju, Republic of Korea. The isolates were catalase-positive and oxidase-negative. Cells of ON8(T) and ON6(T) grew at 15-35 degrees C (optimal 30 degrees C) and 15-40 degrees C (optimal 30 degrees C), respectively. The major menaquinone was MK-7 and the major cellular fatty acids (> 10% of the total) were anteiso-C-15 : 0, iso-C-15 : 0, C-14 : 0 and C-16 : 0. The predominant polar lipids were diphosphatidylglycerol, aminophospholipid and phospholipid. Meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C contents of strains ON8(T) and ON6(T) were 50.6 and 53.5 mol%, respectively, and the 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbour of both strains was Gorillibacterium massiliense G5(T) (93.9 %), followed by the members of the genus Paenibacillus in the family Paenibacillaceae. The DNA-DNA hybridization relatedness value between ON8(T) and ON6(T) was 44.1 %, which indicated that they represented distinct species. Based on polyphasic characteristics, a novel genus is proposed with the name Paludirhabdus gen. nov., which consists of two species, Paludirhabdus telluriireducens sp. nov. (the type species; type strain ON8(T) = KACC 19267(T) = JCM 31958(T)) and Paludirhabdus pumila sp. nov. (type strain ON6(T) = KACC 19266(T) = JCM 31957(T)).