Site-directed spin labelling of proteins by Suzuki-Miyaura coupling via a genetically encoded aryliodide amino acid

被引：15

作者：

Kugele, Anandi ^{[1
,2
]}

Braun, Theresa Sophie ^{[1
,2
]}

Widder, Pia ^{[1
,2
]}

Williams, Lara ^{[1
,2
]}

Schmidt, Moritz Johannes ^{[1
,2
]}

Summerer, Daniel ^{[3
]}

Drescher, Malte ^{[1
,2
]}

机构：

[1] Univ Konstanz, Dept Chem, Univ Str 10, D-78457 Constance, Germany

[2] Univ Konstanz, Konstanz Res Sch Chem Biol KoRS CB, Univ Str 10, D-78457 Constance, Germany

[3] TU Dortmund Univ, Fac Chem & Chem Biol, Otto Hahn Str 4a, D-44227 Dortmund, Germany

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 13期

基金：

欧洲研究理事会;

关键词：

ESCHERICHIA-COLI; ALPHA-SYNUCLEIN; IN-VITRO; CHEMISTRY; DISEASE;

D O I：

10.1039/c8cc09325c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report site-directed protein spin labelling via Suzuki-Miyaura coupling of a nitroxide boronic acid label with the genetically encoded amino acid 4-iodo-L-phenylalanine. The resulting spin label bears a rigid biphenyl linkage with lower flexibility than spin label R1. It is suitable to obtain defined electron paramagnetic resonance distance distributions and to report protein-membrane interactions and conformational transitions of alpha-synuclein.

引用

页码：1923 / 1926

页数：4

共 42 条

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