Conformational Dynamics of DNA Polymerases Revealed at the Single-Molecule Level

被引：6
|
作者：
Millar, David P. [1 ]
机构：
[1] Scripps Res Inst, Dept Integrat Struct & Computat Biol, La Jolla, CA 92037 USA
来源：
FRONTIERS IN MOLECULAR BIOSCIENCES | 2022年 / 9卷
关键词：
DNA polymerase; single-molecule FRET; conformational dynamics; DNA replication fidelity; DNA-protein interactions; TRANSLOCATION STEP; SUBSTRATE-BINDING; KLENOW FRAGMENT; REPLICATION; FIDELITY; MECHANISM; FLUORESCENCE; MACHINERY; LANDSCAPE; COMPLEXES;
D O I：
10.3389/fmolb.2022.826593
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA polymerases are intrinsically dynamic macromolecular machines. The purpose of this review is to describe the single-molecule Forster resonance energy transfer (smFRET) methods that are used to probe the conformational dynamics of DNA polymerases, focusing on E. coli DNA polymerase I. The studies reviewed here reveal the conformational dynamics underpinning the nucleotide selection, proofreading and 5 ' nuclease activities of Pol I. Moreover, the mechanisms revealed for Pol I are likely employed across the DNA polymerase family. smFRET methods have also been used to examine other aspects of DNA polymerase activity.
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页数：15
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