The toxic effects of pentachlorophenol on rat Sertoli cells in vitro

被引：26
|
作者：
Yang, SZ
Han, XD [1 ]
Wei, C
Chen, JX
Yin, DQ
机构：
[1] Nanjing Univ, Sch Med, Immunol & Reprod Biol Lab, Nanjing 210093, Peoples R China
[2] Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210093, Peoples R China
来源：
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2005年 / 20卷 / 01期
基金：
中国国家自然科学基金;
关键词：
pentachlorophenol; toxic; Sertoli cell; viability; cytotoxicity; necrosis;
D O I：
10.1016/j.etap.2004.12.060
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Pentachlorophenol (PCP) is the most toxic contaminant of chlorophenols (CPs). Due to improper disposal, PCP has become an environmental pollutant and is now considered to be ubiquitous. Previous studies about the influences of PCP on reproductive function were mostly focused on experiments in vivo. The aim of our present study was to estimate the toxic effects of PCP on cultured Sertoli cells from Sprague-Dawley rats. The viability of Sertoli cells was detected and morphological examination was performed followed by flow cytometric assay to evaluate its toxic effects. The 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay showed that the toxic effects of PCP on cultured Sertoli cells were dose- and time-dependent. By flow cytometric assay, it was found that the number of dead Sertoli cells increased with the increase of exposed PCP levels. The results indicated that PCP had direct and dose-dependent cytotoxic effects on Sertoli cells in vitro.(c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：182 / 187
页数：6
相关论文
共 50 条
  • [31] SURFACE-ASSOCIATED GLYCOSYLTRANSFERASE ACTIVITIES IN RAT SERTOLI CELLS IN-VITRO
    RAYCHOUDHURY, SS
    MILLETTE, CF
    MOLECULAR REPRODUCTION AND DEVELOPMENT, 1993, 36 (02) : 195 - 202
  • [32] Wnt Pathway-Mediated Nano TiO2-Induced Toxic Effects on Rat Primary Cultured Sertoli Cells
    Hong, Fashui
    Zhou, Yingjun
    Ye, Lingqun
    Ze, Yuguan
    Ji, Jianhui
    Zhuang, Juan
    Wang, Ling
    JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2018, 14 (12) : 2124 - 2134
  • [33] Effects of 13 developmentally toxic chemicals on the migration of rat cephalic neural crest cells in vitro
    Usami, Makoto
    Mitsunaga, Katsuyoshi
    Miyajima, Atsuko
    Takamatu, Mina
    Kazama, Shugo
    Irie, Tomohiko
    Doi, Osamu
    Takizawa, Tatsuya
    CONGENITAL ANOMALIES, 2016, 56 (02) : 52 - 59
  • [34] Regulation of α2-macroglobulin expression in rat Sertoli cells and hepatocytes by germ cells in vitro
    Braghiroli, L
    Silvestrini, B
    Sorrentino, C
    Grima, J
    Mruk, D
    Cheng, CY
    BIOLOGY OF REPRODUCTION, 1998, 59 (01) : 111 - 123
  • [35] Deoxynivalenol and Zearalenone: Different Mycotoxins with Different Toxic Effects in the Sertoli Cells of Equus asinus
    Song, Jun-Lin
    Zhang, Guo-Liang
    CELLS, 2021, 10 (08)
  • [36] Characterization of chicken Sertoli cells in vitro
    Guibert, E.
    Briere, S.
    Pelletier, R.
    Brillard, J. P.
    Froment, P.
    POULTRY SCIENCE, 2011, 90 (06) : 1276 - 1286
  • [37] IN VITRO CULTIVATION OF MAMMALIAN SERTOLI CELLS
    KODANI, M
    KODANI, K
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1966, 56 (04) : 1200 - &
  • [38] Toxic effects of tert-butanol on rat fibroblasts in vitro
    Fontana, Luca
    Bergamaschi, Antonio
    Iavicoli, Ivo
    TOXICOLOGY LETTERS, 2009, 189 : S263 - S263
  • [39] The toxic effects of microcystin-LR on rat spermatogonia in vitro
    Zhou, Yuan
    Yuan, Jintao
    Wu, Jiang
    Han, Xiaodong
    TOXICOLOGY LETTERS, 2012, 212 (01) : 48 - 56
  • [40] effects of p,p'-DDE on the intracellular ERK MAPK signal transduction pathway in Rat Sertoli Cells cultured in vitro
    Wang Cong
    Liu H K
    Liu G H
    Song M F
    Liu S H
    Yang Ke-di
    PROCEEDINGS OF THE 3RD INTERNATIONAL ACADEMIC CONFERENCE ON ENVIRONMENTAL AND OCCUPATIONAL MEDICINE, 2006, : 153 - 156
12345