The toxic effects of pentachlorophenol on rat Sertoli cells in vitro

被引：26
|
作者：
Yang, SZ
Han, XD [1 ]
Wei, C
Chen, JX
Yin, DQ
机构：
[1] Nanjing Univ, Sch Med, Immunol & Reprod Biol Lab, Nanjing 210093, Peoples R China
[2] Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210093, Peoples R China
来源：
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2005年 / 20卷 / 01期
基金：
中国国家自然科学基金;
关键词：
pentachlorophenol; toxic; Sertoli cell; viability; cytotoxicity; necrosis;
D O I：
10.1016/j.etap.2004.12.060
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Pentachlorophenol (PCP) is the most toxic contaminant of chlorophenols (CPs). Due to improper disposal, PCP has become an environmental pollutant and is now considered to be ubiquitous. Previous studies about the influences of PCP on reproductive function were mostly focused on experiments in vivo. The aim of our present study was to estimate the toxic effects of PCP on cultured Sertoli cells from Sprague-Dawley rats. The viability of Sertoli cells was detected and morphological examination was performed followed by flow cytometric assay to evaluate its toxic effects. The 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay showed that the toxic effects of PCP on cultured Sertoli cells were dose- and time-dependent. By flow cytometric assay, it was found that the number of dead Sertoli cells increased with the increase of exposed PCP levels. The results indicated that PCP had direct and dose-dependent cytotoxic effects on Sertoli cells in vitro.(c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：182 / 187
页数：6
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