Superoxide scavenging activities of sixty Chinese medicines determined by an ESR spin-trapping method using electrogenerated superoxide

Superoxide-scavenging activities of 60 kinds of Chinese herbal medicines were determined accurately by an electron spin resonance (ESR) spin-trapping technique using 5,5-dimethyl-1-pyrroline 1-oxide (DMPO), as a spin-trapping reagent. As a source of superoxide in this method, superoxide generated by one-electron reduction of the oxygen molecule in dimethyl sulfoxide solution was used. As a result of these studies, very powerful scavenging activity was found in Chinese medicines for inflammation, diseases of blood circulation and for tumors.