Production and characterization of biosurfactants from Bacillus licheniformis F2.2

被引:83
|
作者
Thaniyavarn, J [1 ]
Roongsawang, N
Kameyama, T
Haruki, M
Imanaka, T
Morikawa, M
Kanaya, S
机构
[1] Chulalongkorn Univ, Fac Sci, Dept Microbiol, Bangkok 10330, Thailand
[2] Osaka Univ, Grad Sch Engn, Dept Mat & Life Sci, Suita, Osaka 5650871, Japan
[3] Kyoto Univ, Grad Sch Engn, Dept Synth Chem & Biol Chem, Kyoto 6868501, Japan
关键词
Bacillus licheniformis; biosurfactant(s); lipopeptide(s); plipastatin; surfactin;
D O I
10.1271/bbb.67.1239
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A biosurfactant-producing strain, Bacillus licheniformis F2.2, was isolated from a fermented food in Thailand. The strain was capable of producing a new biosurfactant, BL1193, as well as two kinds of popular lipopeptide biosurfactants, plipastatin and surfactin. Mass spectrometry and FT-IR analysis indicated that BL1193 had a molecular mass of 1,193 Da with no peptide portion in the molecule. While plipastatin and surfactin were abundantly produced in a nutrient YPD medium, BL1193 was produced only in a synthetic DF medium containing no amino acids. According to an oil displacement activity test, the specific activity of BL1193 (6.53 kBS units/mg) is equivalent to that of surfactin (5.78-6.83 kBS units/mg).
引用
收藏
页码:1239 / 1244
页数:6
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