A metagenomic approach to the identification of UDP-glucose 4-epimerase as a menadione resistance protein

被引：8

作者：

Mori, Toshio ^{[1
]}

Suenaga, Hikaru ^{[1
]}

Miyazaki, Kentaro ^{[1
,2
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Inst Biol Resources & Funct, Tsukuba, Ibaraki 3058566, Japan

[2] Univ Tokyo, Grad Sch Frontier Sci, Dept Med Genome Sci, Tsukuba, Ibaraki 3058566, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2008年 / 72卷 / 06期

关键词：

metagenome; menadione; quinone; reactive oxygen species; UDP-glucose; 4-epimerase;

D O I：

10.1271/bbb.70815

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Quinones are potentially toxic agents that generate reactive oxygen species (ROS) upon reduction. We screened a metagenomic library for a menadione resistance gene to identify UDP-glucose 4-epimerase (UGE). Escherichia coli carrying the gene became resistant to various quinones, but not to ROS. Because UGE is involved in the biosynthesis of lipopolysaccharides, it may have contributed to forming a permeability barrier against lipophilic quinones.

引用

页码：1611 / 1614

页数：4

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