Hymenobacter translucens sp. nov. and Hymenobacter pini sp. nov. isolated from soil

Two novel bacterial strains BT175(T) and BT728(T) were isolated from soil collected in the Republic of Korea. Both strains were Gram stain negative, rod shaped, and had circular, convex, and pink-colored colonies. The 16S rRNA gene sequence similarity between the strains BT175(T) and BT728(T) was 93.6%, indicating that they represent a distinct species. 16S rRNA sequence analysis indicated that strains BT175(T) and BT728(T) belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia, phylum Bacteroidetes). Strain BT175(T) was closely related to Hymenobacter persicinus 1-3-3-3(T) (97.2%, 16S rRNA gene similarity), Hymenobacter knuensis 16F7C-2(T) (96.6%), and Hymenobacter daejeonensis PB105(T) (96.1%). Strain BT728(T) was closely related to Hymenobacter rigui KCTC 12533(T) (98.4%), Hymenobacter metallilatus 9PBR-2(T) (97.6%), and Hymenobacter perfusus LMG26000(T) (97.4%). Strain BT175(T) and BT728(T) were found to have the MK-7 as the major respiratory quinone. The DNA G + C content of strain BT175(T) was 61.6 mol% and BT728(T) was 59.6 mol%, respectively. Characterization based on polyphasic analysis indicated that strains BT175(T) and BT728(T) represent novel species of the genus Hymenobacter and the names Hymenobacter translucens sp. nov. and Hymenobacter pini sp. nov. are proposed. The type strain of Hymenobacter translucens is BT175(T) (= KCTC 72330(T) = NBRC 115441(T)) and Hymenobacter pini is BT728(T) (= KACC 22629(T) = NBRC 115444(T)).