Microbial Conversion of Crude Glycerol to Dihydroxyacetone

被引:12
|
作者
Liebminger, Stefan [1 ]
Hofbauer, Robert [2 ]
Siebenhofer, Matthaeus [3 ]
Nyanhongo, Gibson S. [2 ]
Guebitz, Georg M. [2 ]
机构
[1] Res Ctr Appl Biocatalysis, Graz, Austria
[2] Univ Nat Resources & Life Sci, Inst Environm Biotechnol, A-3430 Tulln, Austria
[3] VTU Engn GmbH, Grambach, Austria
关键词
Crude glycerol; Bioreactor; Dihydroxyacetone; Foam separation technique; Reverse osmosis technique; GLUCONOBACTER-OXYDANS; FOAM FRACTIONATION; RAW GLYCEROL; ACID; OPTIMIZATION; PURIFICATION; SEPARATION; ADSORPTION; PROTEINS; KINETICS;
D O I
10.1007/s12649-013-9288-x
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Glycerol is increasingly being used as a versatile raw material within the biorefinery. However, it is very expensive to refine the crude glycerol to a high purity. In this study, the biotransformation of crude glycerol (79 % purity) using Gluconobacter oxydans spp. oxydans for the production of fine chemical dihydroxyacetone (DHA) was investigated. A conversion of up to 90 % with crude glycerol was demonstrated when using sorbitol as a secondary carbon source to enhance biomass production. A novel process for the recovery of DHA employing generation of foam, filtration and reverse osmosis, was developed. Using this strategy the protein content was reduced by up to 80 % and DHA recovery higher than 94 %.
引用
收藏
页码:781 / 787
页数:7
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