2′-Aminoethoxy-2-amino-3-methylpyridine in Triplex-Forming Oligonucleotides: High Affinity, Selectivity and Resistance to Enzymatic Degradation

被引：9
|
作者：
Lou, Chenguang [1 ]
Shelbourne, Montserrat [1 ]
Fox, Keith R. [2 ]
Brown, Tom [1 ]
机构：
[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England
[2] Univ Southampton, Ctr Biol Sci, Southampton SO17 1BJ, Hants, England
来源：
CHEMISTRY-A EUROPEAN JOURNAL | 2011年 / 17卷 / 52期
基金：
英国生物技术与生命科学研究理事会;
关键词：
DNA structures; nuclease resistance; nucleosides; oligonucleotides; DOUBLE-STRANDED DNA; DUAL RECOGNITION; HELIX FORMATION; SEQUENCE;
D O I：
10.1002/chem.201102287
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The phosphoramidite monomer of the C-nucleoside 2'-aminoethoxy-2-amino-3-methylpyridine (AE-MAP) has been synthesized for the first time and incorporated into triplex-forming oligonucleotides (TFOs). Ultraviolet melting and DNase I footprinting studies show that AE-MAP is a potent triplex-stabilizing monomer that is selective for GC base pairs. TFOs containing AE-MAP bind with high affinity to duplexes but only weakly to single stranded DNA. In addition, AE-MAP confers high nuclease resistance on oligonucleotides. TFOs containing AE-MAP have potential for gene knock-out and gene expression studies.
引用
收藏
页码：14851 / 14856
页数：6
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