2′-Aminoethoxy-2-amino-3-methylpyridine in Triplex-Forming Oligonucleotides: High Affinity, Selectivity and Resistance to Enzymatic Degradation

被引：9

作者：

Lou, Chenguang ^{[1
]}

Shelbourne, Montserrat ^{[1
]}

Fox, Keith R. ^{[2
]}

Brown, Tom ^{[1
]}

机构：

[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England

[2] Univ Southampton, Ctr Biol Sci, Southampton SO17 1BJ, Hants, England

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2011年 / 17卷 / 52期

基金：

英国生物技术与生命科学研究理事会;

关键词：

DNA structures; nuclease resistance; nucleosides; oligonucleotides; DOUBLE-STRANDED DNA; DUAL RECOGNITION; HELIX FORMATION; SEQUENCE;

D O I：

10.1002/chem.201102287

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The phosphoramidite monomer of the C-nucleoside 2'-aminoethoxy-2-amino-3-methylpyridine (AE-MAP) has been synthesized for the first time and incorporated into triplex-forming oligonucleotides (TFOs). Ultraviolet melting and DNase I footprinting studies show that AE-MAP is a potent triplex-stabilizing monomer that is selective for GC base pairs. TFOs containing AE-MAP bind with high affinity to duplexes but only weakly to single stranded DNA. In addition, AE-MAP confers high nuclease resistance on oligonucleotides. TFOs containing AE-MAP have potential for gene knock-out and gene expression studies.

引用

页码：14851 / 14856

页数：6

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